Job ID = 14519808 SRX = SRX9786271 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9193598 spots for SRR13362119/SRR13362119.sra Written 9193598 spots for SRR13362119/SRR13362119.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:32 9193598 reads; of these: 9193598 (100.00%) were paired; of these: 864277 (9.40%) aligned concordantly 0 times 7001910 (76.16%) aligned concordantly exactly 1 time 1327411 (14.44%) aligned concordantly >1 times ---- 864277 pairs aligned concordantly 0 times; of these: 8981 (1.04%) aligned discordantly 1 time ---- 855296 pairs aligned 0 times concordantly or discordantly; of these: 1710592 mates make up the pairs; of these: 1120034 (65.48%) aligned 0 times 486856 (28.46%) aligned exactly 1 time 103702 (6.06%) aligned >1 times 93.91% overall alignment rate Time searching: 00:11:32 Overall time: 00:11:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 766446 / 8337442 = 0.0919 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:21: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:08:34: 2000000 INFO @ Sat, 15 Jan 2022 18:08:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:08:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:08:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:08:46: 3000000 INFO @ Sat, 15 Jan 2022 18:08:49: 1000000 INFO @ Sat, 15 Jan 2022 18:09:00: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:09:04: 2000000 INFO @ Sat, 15 Jan 2022 18:09:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:09:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:09:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:09:14: 5000000 INFO @ Sat, 15 Jan 2022 18:09:17: 3000000 INFO @ Sat, 15 Jan 2022 18:09:17: 1000000 INFO @ Sat, 15 Jan 2022 18:09:28: 6000000 INFO @ Sat, 15 Jan 2022 18:09:29: 2000000 INFO @ Sat, 15 Jan 2022 18:09:31: 4000000 INFO @ Sat, 15 Jan 2022 18:09:40: 3000000 INFO @ Sat, 15 Jan 2022 18:09:42: 7000000 INFO @ Sat, 15 Jan 2022 18:09:45: 5000000 INFO @ Sat, 15 Jan 2022 18:09:51: 4000000 INFO @ Sat, 15 Jan 2022 18:09:55: 8000000 INFO @ Sat, 15 Jan 2022 18:09:59: 6000000 INFO @ Sat, 15 Jan 2022 18:10:02: 5000000 INFO @ Sat, 15 Jan 2022 18:10:10: 9000000 INFO @ Sat, 15 Jan 2022 18:10:12: 7000000 INFO @ Sat, 15 Jan 2022 18:10:13: 6000000 INFO @ Sat, 15 Jan 2022 18:10:24: 10000000 INFO @ Sat, 15 Jan 2022 18:10:25: 7000000 INFO @ Sat, 15 Jan 2022 18:10:25: 8000000 INFO @ Sat, 15 Jan 2022 18:10:37: 8000000 INFO @ Sat, 15 Jan 2022 18:10:38: 11000000 INFO @ Sat, 15 Jan 2022 18:10:39: 9000000 INFO @ Sat, 15 Jan 2022 18:10:48: 9000000 INFO @ Sat, 15 Jan 2022 18:10:52: 12000000 INFO @ Sat, 15 Jan 2022 18:10:54: 10000000 INFO @ Sat, 15 Jan 2022 18:10:59: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:11:06: 13000000 INFO @ Sat, 15 Jan 2022 18:11:08: 11000000 INFO @ Sat, 15 Jan 2022 18:11:11: 11000000 INFO @ Sat, 15 Jan 2022 18:11:20: 14000000 INFO @ Sat, 15 Jan 2022 18:11:22: 12000000 INFO @ Sat, 15 Jan 2022 18:11:23: 12000000 INFO @ Sat, 15 Jan 2022 18:11:34: 13000000 INFO @ Sat, 15 Jan 2022 18:11:35: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:11:36: 13000000 INFO @ Sat, 15 Jan 2022 18:11:43: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:11:43: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:11:43: #1 total tags in treatment: 7563021 INFO @ Sat, 15 Jan 2022 18:11:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:11:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:11:44: #1 tags after filtering in treatment: 4244597 INFO @ Sat, 15 Jan 2022 18:11:44: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:11:44: #1 finished! INFO @ Sat, 15 Jan 2022 18:11:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:11:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:11:44: #2 number of paired peaks: 2 WARNING @ Sat, 15 Jan 2022 18:11:44: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:11:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:11:45: 14000000 INFO @ Sat, 15 Jan 2022 18:11:50: 14000000 INFO @ Sat, 15 Jan 2022 18:11:57: 15000000 INFO @ Sat, 15 Jan 2022 18:12:02: 15000000 INFO @ Sat, 15 Jan 2022 18:12:05: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:12:06: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:12:06: #1 total tags in treatment: 7563021 INFO @ Sat, 15 Jan 2022 18:12:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:06: #1 tags after filtering in treatment: 4244597 INFO @ Sat, 15 Jan 2022 18:12:06: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:12:06: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:06: #2 number of paired peaks: 2 WARNING @ Sat, 15 Jan 2022 18:12:06: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:12:10: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:12:10: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:12:10: #1 total tags in treatment: 7563021 INFO @ Sat, 15 Jan 2022 18:12:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:12:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:12:10: #1 tags after filtering in treatment: 4244597 INFO @ Sat, 15 Jan 2022 18:12:10: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:12:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:12:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:12:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:12:11: #2 number of paired peaks: 2 WARNING @ Sat, 15 Jan 2022 18:12:11: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:12:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9786271/SRX9786271.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling