Job ID = 11193225 sra ファイルのダウンロード中... Completed: 347853K bytes transferred in 6 seconds (434276K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 8510513 spots for /home/okishinya/chipatlas/results/sacCer3/SRX977511/SRR1951357.sra Written 8510513 spots for /home/okishinya/chipatlas/results/sacCer3/SRX977511/SRR1951357.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:00 8510513 reads; of these: 8510513 (100.00%) were paired; of these: 4905064 (57.64%) aligned concordantly 0 times 2979850 (35.01%) aligned concordantly exactly 1 time 625599 (7.35%) aligned concordantly >1 times ---- 4905064 pairs aligned concordantly 0 times; of these: 191954 (3.91%) aligned discordantly 1 time ---- 4713110 pairs aligned 0 times concordantly or discordantly; of these: 9426220 mates make up the pairs; of these: 9113643 (96.68%) aligned 0 times 177486 (1.88%) aligned exactly 1 time 135091 (1.43%) aligned >1 times 46.46% overall alignment rate Time searching: 00:03:00 Overall time: 00:03:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 262790 / 3751290 = 0.0701 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:18:22: # Command line: callpeak -t SRX977511.bam -f BAM -g 12100000 -n SRX977511.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX977511.05 # format = BAM # ChIP-seq file = ['SRX977511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:18:22: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:18:22: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:18:22: # Command line: callpeak -t SRX977511.bam -f BAM -g 12100000 -n SRX977511.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX977511.10 # format = BAM # ChIP-seq file = ['SRX977511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:18:22: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:18:22: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:18:22: # Command line: callpeak -t SRX977511.bam -f BAM -g 12100000 -n SRX977511.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX977511.20 # format = BAM # ChIP-seq file = ['SRX977511.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:18:22: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:18:22: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:18:28: 1000000 INFO @ Sat, 15 Sep 2018 11:18:28: 1000000 INFO @ Sat, 15 Sep 2018 11:18:28: 1000000 INFO @ Sat, 15 Sep 2018 11:18:33: 2000000 INFO @ Sat, 15 Sep 2018 11:18:33: 2000000 INFO @ Sat, 15 Sep 2018 11:18:33: 2000000 INFO @ Sat, 15 Sep 2018 11:18:39: 3000000 INFO @ Sat, 15 Sep 2018 11:18:39: 3000000 INFO @ Sat, 15 Sep 2018 11:18:39: 3000000 INFO @ Sat, 15 Sep 2018 11:18:45: 4000000 INFO @ Sat, 15 Sep 2018 11:18:45: 4000000 INFO @ Sat, 15 Sep 2018 11:18:45: 4000000 INFO @ Sat, 15 Sep 2018 11:18:50: 5000000 INFO @ Sat, 15 Sep 2018 11:18:51: 5000000 INFO @ Sat, 15 Sep 2018 11:18:51: 5000000 INFO @ Sat, 15 Sep 2018 11:18:56: 6000000 INFO @ Sat, 15 Sep 2018 11:18:57: 6000000 INFO @ Sat, 15 Sep 2018 11:18:57: 6000000 INFO @ Sat, 15 Sep 2018 11:19:02: 7000000 INFO @ Sat, 15 Sep 2018 11:19:02: 7000000 INFO @ Sat, 15 Sep 2018 11:19:02: 7000000 INFO @ Sat, 15 Sep 2018 11:19:04: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:19:04: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:19:04: #1 total tags in treatment: 3354515 INFO @ Sat, 15 Sep 2018 11:19:04: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:19:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:19:04: #1 tags after filtering in treatment: 2763249 INFO @ Sat, 15 Sep 2018 11:19:04: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 11:19:04: #1 finished! INFO @ Sat, 15 Sep 2018 11:19:04: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:19:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:19:04: #2 number of paired peaks: 31 WARNING @ Sat, 15 Sep 2018 11:19:04: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:19:04: Process for pairing-model is terminated! cat: SRX977511.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX977511.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:19:04: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:19:04: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:19:04: #1 total tags in treatment: 3354515 INFO @ Sat, 15 Sep 2018 11:19:04: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:19:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:19:05: #1 tags after filtering in treatment: 2763249 INFO @ Sat, 15 Sep 2018 11:19:05: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 11:19:05: #1 finished! INFO @ Sat, 15 Sep 2018 11:19:05: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:19:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:19:05: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:19:05: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:19:05: #1 total tags in treatment: 3354515 INFO @ Sat, 15 Sep 2018 11:19:05: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:19:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:19:05: #2 number of paired peaks: 31 WARNING @ Sat, 15 Sep 2018 11:19:05: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:19:05: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 11:19:05: #1 tags after filtering in treatment: 2763249 INFO @ Sat, 15 Sep 2018 11:19:05: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 11:19:05: #1 finished! INFO @ Sat, 15 Sep 2018 11:19:05: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:19:05: #2 looking for paired plus/minus strand peaks... cat: SRX977511.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX977511.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:19:05: #2 number of paired peaks: 31 WARNING @ Sat, 15 Sep 2018 11:19:05: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:19:05: Process for pairing-model is terminated! cat: SRX977511.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX977511.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX977511.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。