Job ID = 9037700 sra ファイルのダウンロード中... Completed: 314063K bytes transferred in 5 seconds (441542K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- 0:00:06 --:--:-- 0 100 14324 0 14324 0 0 1982 0 --:--:-- 0:00:07 --:--:-- 14352 100 44968 0 44968 0 0 5360 0 --:--:-- 0:00:08 --:--:-- 20828 100 46839 0 46839 0 0 5583 0 --:--:-- 0:00:08 --:--:-- 21684 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 14579127 spots for /home/okishinya/chipatlas/results/sacCer3/SRX977491/SRR1951337.sra Written 14579127 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 14579127 reads; of these: 14579127 (100.00%) were unpaired; of these: 3896903 (26.73%) aligned 0 times 9418710 (64.60%) aligned exactly 1 time 1263514 (8.67%) aligned >1 times 73.27% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 10413468 / 10682224 = 0.9748 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 04 Jun 2017 05:13:53: # Command line: callpeak -t SRX977491.bam -f BAM -g 12100000 -n SRX977491.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX977491.05 # format = BAM # ChIP-seq file = ['SRX977491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:13:53: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:13:53: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:13:53: # Command line: callpeak -t SRX977491.bam -f BAM -g 12100000 -n SRX977491.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX977491.10 # format = BAM # ChIP-seq file = ['SRX977491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:13:53: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:13:53: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:13:53: # Command line: callpeak -t SRX977491.bam -f BAM -g 12100000 -n SRX977491.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX977491.20 # format = BAM # ChIP-seq file = ['SRX977491.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:13:53: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:13:53: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:13:54: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:13:54: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:13:54: #1 total tags in treatment: 268756 INFO @ Sun, 04 Jun 2017 05:13:54: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:13:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:13:55: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:13:55: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:13:55: #1 total tags in treatment: 268756 INFO @ Sun, 04 Jun 2017 05:13:55: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:13:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:13:55: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:13:55: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:13:55: #1 total tags in treatment: 268756 INFO @ Sun, 04 Jun 2017 05:13:55: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:13:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:13:55: #1 tags after filtering in treatment: 268485 INFO @ Sun, 04 Jun 2017 05:13:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:13:55: #1 finished! INFO @ Sun, 04 Jun 2017 05:13:55: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:13:55: #1 tags after filtering in treatment: 268485 INFO @ Sun, 04 Jun 2017 05:13:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:13:55: #1 finished! INFO @ Sun, 04 Jun 2017 05:13:55: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:13:55: #1 tags after filtering in treatment: 268485 INFO @ Sun, 04 Jun 2017 05:13:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:13:55: #1 finished! INFO @ Sun, 04 Jun 2017 05:13:55: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:13:55: #2 number of paired peaks: 596 WARNING @ Sun, 04 Jun 2017 05:13:55: Fewer paired peaks (596) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 596 pairs to build model! INFO @ Sun, 04 Jun 2017 05:13:55: start model_add_line... INFO @ Sun, 04 Jun 2017 05:13:55: #2 number of paired peaks: 596 WARNING @ Sun, 04 Jun 2017 05:13:55: Fewer paired peaks (596) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 596 pairs to build model! INFO @ Sun, 04 Jun 2017 05:13:55: start model_add_line... INFO @ Sun, 04 Jun 2017 05:13:55: #2 number of paired peaks: 596 WARNING @ Sun, 04 Jun 2017 05:13:55: Fewer paired peaks (596) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 596 pairs to build model! INFO @ Sun, 04 Jun 2017 05:13:55: start model_add_line... INFO @ Sun, 04 Jun 2017 05:13:56: start X-correlation... INFO @ Sun, 04 Jun 2017 05:13:56: end of X-cor INFO @ Sun, 04 Jun 2017 05:13:56: #2 finished! INFO @ Sun, 04 Jun 2017 05:13:56: #2 predicted fragment length is 43 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2 alternative fragment length(s) may be 43,432,453,469,525,540,564 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2.2 Generate R script for model : SRX977491.10_model.r WARNING @ Sun, 04 Jun 2017 05:13:56: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You may need to consider one of the other alternative d(s): 43,432,453,469,525,540,564 WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 04 Jun 2017 05:13:56: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:13:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Jun 2017 05:13:56: start X-correlation... INFO @ Sun, 04 Jun 2017 05:13:56: end of X-cor INFO @ Sun, 04 Jun 2017 05:13:56: #2 finished! INFO @ Sun, 04 Jun 2017 05:13:56: #2 predicted fragment length is 43 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2 alternative fragment length(s) may be 43,432,453,469,525,540,564 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2.2 Generate R script for model : SRX977491.20_model.r WARNING @ Sun, 04 Jun 2017 05:13:56: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You may need to consider one of the other alternative d(s): 43,432,453,469,525,540,564 WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 04 Jun 2017 05:13:56: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:13:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Jun 2017 05:13:56: start X-correlation... INFO @ Sun, 04 Jun 2017 05:13:56: end of X-cor INFO @ Sun, 04 Jun 2017 05:13:56: #2 finished! INFO @ Sun, 04 Jun 2017 05:13:56: #2 predicted fragment length is 43 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2 alternative fragment length(s) may be 43,432,453,469,525,540,564 bps INFO @ Sun, 04 Jun 2017 05:13:56: #2.2 Generate R script for model : SRX977491.05_model.r WARNING @ Sun, 04 Jun 2017 05:13:56: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You may need to consider one of the other alternative d(s): 43,432,453,469,525,540,564 WARNING @ Sun, 04 Jun 2017 05:13:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 04 Jun 2017 05:13:56: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:13:56: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Jun 2017 05:13:58: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:13:58: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:13:58: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write output xls file... SRX977491.20_peaks.xls INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write peak in narrowPeak format file... SRX977491.20_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write summits bed file... SRX977491.20_summits.bed INFO @ Sun, 04 Jun 2017 05:13:59: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (44 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write output xls file... SRX977491.10_peaks.xls INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write peak in narrowPeak format file... SRX977491.10_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write summits bed file... SRX977491.10_summits.bed INFO @ Sun, 04 Jun 2017 05:13:59: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (213 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write output xls file... SRX977491.05_peaks.xls INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write peak in narrowPeak format file... SRX977491.05_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:13:59: #4 Write summits bed file... SRX977491.05_summits.bed INFO @ Sun, 04 Jun 2017 05:13:59: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (508 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。