Job ID = 9037673 sra ファイルのダウンロード中... Completed: 21203K bytes transferred in 3 seconds (54280K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- 0:00:06 --:--:-- 0 0 0 0 0 0 0 0 0 --:--:-- 0:00:07 --:--:-- 0 100 13455 0 13455 0 0 1745 0 --:--:-- 0:00:07 --:--:-- 10255 100 33902 0 33902 0 0 3969 0 --:--:-- 0:00:08 --:--:-- 15827 100 40437 0 40437 0 0 4623 0 --:--:-- 0:00:08 --:--:-- 17214 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 858785 spots for /home/okishinya/chipatlas/results/sacCer3/SRX977464/SRR1951310.sra Written 858785 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:07 858785 reads; of these: 858785 (100.00%) were unpaired; of these: 138219 (16.09%) aligned 0 times 637785 (74.27%) aligned exactly 1 time 82781 (9.64%) aligned >1 times 83.91% overall alignment rate Time searching: 00:00:07 Overall time: 00:00:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 673732 / 720566 = 0.9350 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 04 Jun 2017 05:08:15: # Command line: callpeak -t SRX977464.bam -f BAM -g 12100000 -n SRX977464.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX977464.20 # format = BAM # ChIP-seq file = ['SRX977464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:08:15: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:08:15: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:08:15: # Command line: callpeak -t SRX977464.bam -f BAM -g 12100000 -n SRX977464.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX977464.05 # format = BAM # ChIP-seq file = ['SRX977464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:08:15: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:08:15: # Command line: callpeak -t SRX977464.bam -f BAM -g 12100000 -n SRX977464.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX977464.10 # format = BAM # ChIP-seq file = ['SRX977464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sun, 04 Jun 2017 05:08:15: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:08:15: #1 read tag files... INFO @ Sun, 04 Jun 2017 05:08:15: #1 read treatment tags... INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:08:15: #1 total tags in treatment: 46834 INFO @ Sun, 04 Jun 2017 05:08:15: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:08:15: #1 total tags in treatment: 46834 INFO @ Sun, 04 Jun 2017 05:08:15: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size is determined as 40 bps INFO @ Sun, 04 Jun 2017 05:08:15: #1 tag size = 40 INFO @ Sun, 04 Jun 2017 05:08:15: #1 total tags in treatment: 46834 INFO @ Sun, 04 Jun 2017 05:08:15: #1 user defined the maximum tags... INFO @ Sun, 04 Jun 2017 05:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 04 Jun 2017 05:08:15: #1 tags after filtering in treatment: 46811 INFO @ Sun, 04 Jun 2017 05:08:15: #1 tags after filtering in treatment: 46811 INFO @ Sun, 04 Jun 2017 05:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:08:15: #1 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #1 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:08:15: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:08:15: #1 tags after filtering in treatment: 46811 INFO @ Sun, 04 Jun 2017 05:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 04 Jun 2017 05:08:15: #1 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #2 Build Peak Model... INFO @ Sun, 04 Jun 2017 05:08:15: #2 number of paired peaks: 422 WARNING @ Sun, 04 Jun 2017 05:08:15: Fewer paired peaks (422) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 422 pairs to build model! INFO @ Sun, 04 Jun 2017 05:08:15: start model_add_line... INFO @ Sun, 04 Jun 2017 05:08:15: #2 number of paired peaks: 422 WARNING @ Sun, 04 Jun 2017 05:08:15: Fewer paired peaks (422) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 422 pairs to build model! INFO @ Sun, 04 Jun 2017 05:08:15: start model_add_line... INFO @ Sun, 04 Jun 2017 05:08:15: #2 number of paired peaks: 422 WARNING @ Sun, 04 Jun 2017 05:08:15: Fewer paired peaks (422) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 422 pairs to build model! INFO @ Sun, 04 Jun 2017 05:08:15: start model_add_line... INFO @ Sun, 04 Jun 2017 05:08:15: start X-correlation... INFO @ Sun, 04 Jun 2017 05:08:15: start X-correlation... INFO @ Sun, 04 Jun 2017 05:08:15: start X-correlation... INFO @ Sun, 04 Jun 2017 05:08:15: end of X-cor INFO @ Sun, 04 Jun 2017 05:08:15: #2 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #2 predicted fragment length is 255 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2 alternative fragment length(s) may be 13,52,80,86,122,174,207,255,279,380,463,562 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2.2 Generate R script for model : SRX977464.20_model.r INFO @ Sun, 04 Jun 2017 05:08:15: end of X-cor INFO @ Sun, 04 Jun 2017 05:08:15: #2 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #2 predicted fragment length is 255 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2 alternative fragment length(s) may be 13,52,80,86,122,174,207,255,279,380,463,562 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2.2 Generate R script for model : SRX977464.05_model.r INFO @ Sun, 04 Jun 2017 05:08:15: end of X-cor INFO @ Sun, 04 Jun 2017 05:08:15: #2 finished! INFO @ Sun, 04 Jun 2017 05:08:15: #2 predicted fragment length is 255 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2 alternative fragment length(s) may be 13,52,80,86,122,174,207,255,279,380,463,562 bps INFO @ Sun, 04 Jun 2017 05:08:15: #2.2 Generate R script for model : SRX977464.10_model.r INFO @ Sun, 04 Jun 2017 05:08:15: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:08:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Jun 2017 05:08:15: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:08:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 04 Jun 2017 05:08:15: #3 Call peaks... INFO @ Sun, 04 Jun 2017 05:08:15: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sun, 04 Jun 2017 05:08:16: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:08:16: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:08:16: #3 Call peaks for each chromosome... INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write output xls file... SRX977464.10_peaks.xls INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write peak in narrowPeak format file... SRX977464.10_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write summits bed file... SRX977464.10_summits.bed INFO @ Sun, 04 Jun 2017 05:08:16: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (11 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write output xls file... SRX977464.05_peaks.xls INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write peak in narrowPeak format file... SRX977464.05_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write summits bed file... SRX977464.05_summits.bed INFO @ Sun, 04 Jun 2017 05:08:16: Done! INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write output xls file... SRX977464.20_peaks.xls INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write peak in narrowPeak format file... SRX977464.20_peaks.narrowPeak INFO @ Sun, 04 Jun 2017 05:08:16: #4 Write summits bed file... SRX977464.20_summits.bed INFO @ Sun, 04 Jun 2017 05:08:16: Done! pass1 - making usageList (9 chroms): 0 millis pass2 - checking and writing primary data (22 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (1 records, 4 fields): 1 millis CompletedMACS2peakCalling