Job ID = 14522043 SRX = SRX9773810 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9429856 spots for SRR13347751/SRR13347751.sra Written 9429856 spots for SRR13347751/SRR13347751.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:06 9429856 reads; of these: 9429856 (100.00%) were paired; of these: 266975 (2.83%) aligned concordantly 0 times 7775044 (82.45%) aligned concordantly exactly 1 time 1387837 (14.72%) aligned concordantly >1 times ---- 266975 pairs aligned concordantly 0 times; of these: 17600 (6.59%) aligned discordantly 1 time ---- 249375 pairs aligned 0 times concordantly or discordantly; of these: 498750 mates make up the pairs; of these: 386118 (77.42%) aligned 0 times 82900 (16.62%) aligned exactly 1 time 29732 (5.96%) aligned >1 times 97.95% overall alignment rate Time searching: 00:04:06 Overall time: 00:04:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 652458 / 9177738 = 0.0711 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:10:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:10:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:10:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:10:54: 1000000 INFO @ Sat, 15 Jan 2022 22:10:58: 2000000 INFO @ Sat, 15 Jan 2022 22:11:03: 3000000 INFO @ Sat, 15 Jan 2022 22:11:08: 4000000 INFO @ Sat, 15 Jan 2022 22:11:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:18: 6000000 INFO @ Sat, 15 Jan 2022 22:11:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:24: 7000000 INFO @ Sat, 15 Jan 2022 22:11:25: 1000000 INFO @ Sat, 15 Jan 2022 22:11:30: 8000000 INFO @ Sat, 15 Jan 2022 22:11:31: 2000000 INFO @ Sat, 15 Jan 2022 22:11:37: 9000000 INFO @ Sat, 15 Jan 2022 22:11:38: 3000000 INFO @ Sat, 15 Jan 2022 22:11:43: 10000000 INFO @ Sat, 15 Jan 2022 22:11:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:49: 11000000 INFO @ Sat, 15 Jan 2022 22:11:51: 5000000 INFO @ Sat, 15 Jan 2022 22:11:56: 12000000 INFO @ Sat, 15 Jan 2022 22:11:56: 1000000 INFO @ Sat, 15 Jan 2022 22:11:57: 6000000 INFO @ Sat, 15 Jan 2022 22:12:02: 13000000 INFO @ Sat, 15 Jan 2022 22:12:03: 2000000 INFO @ Sat, 15 Jan 2022 22:12:04: 7000000 INFO @ Sat, 15 Jan 2022 22:12:09: 14000000 INFO @ Sat, 15 Jan 2022 22:12:10: 3000000 INFO @ Sat, 15 Jan 2022 22:12:11: 8000000 INFO @ Sat, 15 Jan 2022 22:12:15: 15000000 INFO @ Sat, 15 Jan 2022 22:12:17: 4000000 INFO @ Sat, 15 Jan 2022 22:12:17: 9000000 INFO @ Sat, 15 Jan 2022 22:12:22: 16000000 INFO @ Sat, 15 Jan 2022 22:12:23: 5000000 INFO @ Sat, 15 Jan 2022 22:12:24: 10000000 INFO @ Sat, 15 Jan 2022 22:12:28: 17000000 INFO @ Sat, 15 Jan 2022 22:12:29: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:12:29: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:12:29: #1 total tags in treatment: 8510826 INFO @ Sat, 15 Jan 2022 22:12:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:12:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:12:29: #1 tags after filtering in treatment: 5774870 INFO @ Sat, 15 Jan 2022 22:12:29: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:12:29: #1 finished! INFO @ Sat, 15 Jan 2022 22:12:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:12:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:12:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:12:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:12:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:12:30: 11000000 INFO @ Sat, 15 Jan 2022 22:12:30: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:12:37: 12000000 INFO @ Sat, 15 Jan 2022 22:12:37: 7000000 INFO @ Sat, 15 Jan 2022 22:12:43: 13000000 INFO @ Sat, 15 Jan 2022 22:12:44: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:12:49: 14000000 INFO @ Sat, 15 Jan 2022 22:12:51: 9000000 INFO @ Sat, 15 Jan 2022 22:12:56: 15000000 INFO @ Sat, 15 Jan 2022 22:12:58: 10000000 INFO @ Sat, 15 Jan 2022 22:13:02: 16000000 INFO @ Sat, 15 Jan 2022 22:13:05: 11000000 INFO @ Sat, 15 Jan 2022 22:13:09: 17000000 INFO @ Sat, 15 Jan 2022 22:13:10: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:13:10: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:13:10: #1 total tags in treatment: 8510826 INFO @ Sat, 15 Jan 2022 22:13:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:13:10: #1 tags after filtering in treatment: 5774870 INFO @ Sat, 15 Jan 2022 22:13:10: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:13:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:13:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:13:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:13:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:13:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:13:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:13:11: 12000000 INFO @ Sat, 15 Jan 2022 22:13:18: 13000000 INFO @ Sat, 15 Jan 2022 22:13:24: 14000000 INFO @ Sat, 15 Jan 2022 22:13:30: 15000000 INFO @ Sat, 15 Jan 2022 22:13:36: 16000000 INFO @ Sat, 15 Jan 2022 22:13:43: 17000000 INFO @ Sat, 15 Jan 2022 22:13:44: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:13:44: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:13:44: #1 total tags in treatment: 8510826 INFO @ Sat, 15 Jan 2022 22:13:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:13:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:13:44: #1 tags after filtering in treatment: 5774870 INFO @ Sat, 15 Jan 2022 22:13:44: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:13:44: #1 finished! INFO @ Sat, 15 Jan 2022 22:13:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:13:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:13:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:13:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:13:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773810/SRX9773810.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling