Job ID = 14522040 SRX = SRX9773807 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 36992120 spots for SRR13347748/SRR13347748.sra Written 36992120 spots for SRR13347748/SRR13347748.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:53 36992120 reads; of these: 36992120 (100.00%) were paired; of these: 3534393 (9.55%) aligned concordantly 0 times 27546043 (74.46%) aligned concordantly exactly 1 time 5911684 (15.98%) aligned concordantly >1 times ---- 3534393 pairs aligned concordantly 0 times; of these: 1394190 (39.45%) aligned discordantly 1 time ---- 2140203 pairs aligned 0 times concordantly or discordantly; of these: 4280406 mates make up the pairs; of these: 2800375 (65.42%) aligned 0 times 659238 (15.40%) aligned exactly 1 time 820793 (19.18%) aligned >1 times 96.21% overall alignment rate Time searching: 00:24:53 Overall time: 00:24:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 15123032 / 34808225 = 0.4345 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:45:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:45:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:45:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:45:04: 1000000 INFO @ Sat, 15 Jan 2022 22:45:07: 2000000 INFO @ Sat, 15 Jan 2022 22:45:11: 3000000 INFO @ Sat, 15 Jan 2022 22:45:15: 4000000 INFO @ Sat, 15 Jan 2022 22:45:19: 5000000 INFO @ Sat, 15 Jan 2022 22:45:23: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:45:27: 7000000 INFO @ Sat, 15 Jan 2022 22:45:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:45:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:45:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:45:31: 8000000 INFO @ Sat, 15 Jan 2022 22:45:34: 1000000 INFO @ Sat, 15 Jan 2022 22:45:36: 9000000 INFO @ Sat, 15 Jan 2022 22:45:39: 2000000 INFO @ Sat, 15 Jan 2022 22:45:40: 10000000 INFO @ Sat, 15 Jan 2022 22:45:43: 3000000 INFO @ Sat, 15 Jan 2022 22:45:44: 11000000 INFO @ Sat, 15 Jan 2022 22:45:47: 4000000 INFO @ Sat, 15 Jan 2022 22:45:49: 12000000 INFO @ Sat, 15 Jan 2022 22:45:52: 5000000 INFO @ Sat, 15 Jan 2022 22:45:53: 13000000 INFO @ Sat, 15 Jan 2022 22:45:56: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:45:58: 14000000 INFO @ Sat, 15 Jan 2022 22:46:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:46:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:46:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:46:01: 7000000 INFO @ Sat, 15 Jan 2022 22:46:02: 15000000 INFO @ Sat, 15 Jan 2022 22:46:05: 1000000 INFO @ Sat, 15 Jan 2022 22:46:06: 8000000 INFO @ Sat, 15 Jan 2022 22:46:07: 16000000 INFO @ Sat, 15 Jan 2022 22:46:09: 2000000 INFO @ Sat, 15 Jan 2022 22:46:11: 9000000 INFO @ Sat, 15 Jan 2022 22:46:12: 17000000 INFO @ Sat, 15 Jan 2022 22:46:14: 3000000 INFO @ Sat, 15 Jan 2022 22:46:16: 10000000 INFO @ Sat, 15 Jan 2022 22:46:17: 18000000 INFO @ Sat, 15 Jan 2022 22:46:19: 4000000 INFO @ Sat, 15 Jan 2022 22:46:21: 11000000 INFO @ Sat, 15 Jan 2022 22:46:22: 19000000 INFO @ Sat, 15 Jan 2022 22:46:24: 5000000 INFO @ Sat, 15 Jan 2022 22:46:26: 12000000 INFO @ Sat, 15 Jan 2022 22:46:27: 20000000 INFO @ Sat, 15 Jan 2022 22:46:29: 6000000 INFO @ Sat, 15 Jan 2022 22:46:31: 13000000 INFO @ Sat, 15 Jan 2022 22:46:32: 21000000 INFO @ Sat, 15 Jan 2022 22:46:34: 7000000 INFO @ Sat, 15 Jan 2022 22:46:36: 14000000 INFO @ Sat, 15 Jan 2022 22:46:37: 22000000 INFO @ Sat, 15 Jan 2022 22:46:39: 8000000 INFO @ Sat, 15 Jan 2022 22:46:41: 15000000 INFO @ Sat, 15 Jan 2022 22:46:42: 23000000 INFO @ Sat, 15 Jan 2022 22:46:44: 9000000 INFO @ Sat, 15 Jan 2022 22:46:46: 16000000 INFO @ Sat, 15 Jan 2022 22:46:47: 24000000 INFO @ Sat, 15 Jan 2022 22:46:49: 10000000 INFO @ Sat, 15 Jan 2022 22:46:51: 17000000 INFO @ Sat, 15 Jan 2022 22:46:52: 25000000 INFO @ Sat, 15 Jan 2022 22:46:54: 11000000 INFO @ Sat, 15 Jan 2022 22:46:56: 18000000 INFO @ Sat, 15 Jan 2022 22:46:57: 26000000 INFO @ Sat, 15 Jan 2022 22:46:59: 12000000 INFO @ Sat, 15 Jan 2022 22:47:01: 19000000 INFO @ Sat, 15 Jan 2022 22:47:02: 27000000 INFO @ Sat, 15 Jan 2022 22:47:04: 13000000 INFO @ Sat, 15 Jan 2022 22:47:06: 20000000 INFO @ Sat, 15 Jan 2022 22:47:06: 28000000 INFO @ Sat, 15 Jan 2022 22:47:09: 14000000 INFO @ Sat, 15 Jan 2022 22:47:11: 21000000 INFO @ Sat, 15 Jan 2022 22:47:11: 29000000 INFO @ Sat, 15 Jan 2022 22:47:13: 15000000 INFO @ Sat, 15 Jan 2022 22:47:16: 22000000 INFO @ Sat, 15 Jan 2022 22:47:16: 30000000 INFO @ Sat, 15 Jan 2022 22:47:18: 16000000 INFO @ Sat, 15 Jan 2022 22:47:21: 23000000 INFO @ Sat, 15 Jan 2022 22:47:21: 31000000 INFO @ Sat, 15 Jan 2022 22:47:23: 17000000 INFO @ Sat, 15 Jan 2022 22:47:26: 24000000 INFO @ Sat, 15 Jan 2022 22:47:26: 32000000 INFO @ Sat, 15 Jan 2022 22:47:28: 18000000 INFO @ Sat, 15 Jan 2022 22:47:31: 25000000 INFO @ Sat, 15 Jan 2022 22:47:31: 33000000 INFO @ Sat, 15 Jan 2022 22:47:33: 19000000 INFO @ Sat, 15 Jan 2022 22:47:36: 26000000 INFO @ Sat, 15 Jan 2022 22:47:36: 34000000 INFO @ Sat, 15 Jan 2022 22:47:38: 20000000 INFO @ Sat, 15 Jan 2022 22:47:40: 27000000 INFO @ Sat, 15 Jan 2022 22:47:41: 35000000 INFO @ Sat, 15 Jan 2022 22:47:43: 21000000 INFO @ Sat, 15 Jan 2022 22:47:45: 28000000 INFO @ Sat, 15 Jan 2022 22:47:46: 36000000 INFO @ Sat, 15 Jan 2022 22:47:48: 22000000 INFO @ Sat, 15 Jan 2022 22:47:50: 29000000 INFO @ Sat, 15 Jan 2022 22:47:51: 37000000 INFO @ Sat, 15 Jan 2022 22:47:53: 23000000 INFO @ Sat, 15 Jan 2022 22:47:55: 30000000 INFO @ Sat, 15 Jan 2022 22:47:56: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:47:58: 24000000 INFO @ Sat, 15 Jan 2022 22:48:00: 31000000 INFO @ Sat, 15 Jan 2022 22:48:01: 39000000 INFO @ Sat, 15 Jan 2022 22:48:02: 25000000 INFO @ Sat, 15 Jan 2022 22:48:05: 32000000 INFO @ Sat, 15 Jan 2022 22:48:06: 40000000 INFO @ Sat, 15 Jan 2022 22:48:07: 26000000 INFO @ Sat, 15 Jan 2022 22:48:10: 33000000 INFO @ Sat, 15 Jan 2022 22:48:11: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:48:11: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:48:11: #1 total tags in treatment: 18698623 INFO @ Sat, 15 Jan 2022 22:48:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:48:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:48:11: #1 tags after filtering in treatment: 10957215 INFO @ Sat, 15 Jan 2022 22:48:11: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:48:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:48:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:48:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:48:12: 27000000 INFO @ Sat, 15 Jan 2022 22:48:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:48:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:48:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:48:15: 34000000 INFO @ Sat, 15 Jan 2022 22:48:16: 28000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:48:19: 35000000 INFO @ Sat, 15 Jan 2022 22:48:20: 29000000 INFO @ Sat, 15 Jan 2022 22:48:24: 36000000 INFO @ Sat, 15 Jan 2022 22:48:25: 30000000 INFO @ Sat, 15 Jan 2022 22:48:28: 37000000 INFO @ Sat, 15 Jan 2022 22:48:29: 31000000 INFO @ Sat, 15 Jan 2022 22:48:32: 38000000 INFO @ Sat, 15 Jan 2022 22:48:33: 32000000 INFO @ Sat, 15 Jan 2022 22:48:37: 39000000 INFO @ Sat, 15 Jan 2022 22:48:38: 33000000 INFO @ Sat, 15 Jan 2022 22:48:41: 40000000 INFO @ Sat, 15 Jan 2022 22:48:42: 34000000 INFO @ Sat, 15 Jan 2022 22:48:46: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:48:46: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:48:46: #1 total tags in treatment: 18698623 INFO @ Sat, 15 Jan 2022 22:48:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:48:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:48:46: #1 tags after filtering in treatment: 10957215 INFO @ Sat, 15 Jan 2022 22:48:46: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:48:46: #1 finished! INFO @ Sat, 15 Jan 2022 22:48:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:48:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:48:47: 35000000 INFO @ Sat, 15 Jan 2022 22:48:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:48:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:48:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:48:51: 36000000 INFO @ Sat, 15 Jan 2022 22:48:55: 37000000 INFO @ Sat, 15 Jan 2022 22:48:59: 38000000 INFO @ Sat, 15 Jan 2022 22:49:03: 39000000 INFO @ Sat, 15 Jan 2022 22:49:07: 40000000 INFO @ Sat, 15 Jan 2022 22:49:10: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:49:10: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:49:10: #1 total tags in treatment: 18698623 INFO @ Sat, 15 Jan 2022 22:49:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:49:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:49:11: #1 tags after filtering in treatment: 10957215 INFO @ Sat, 15 Jan 2022 22:49:11: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:49:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:49:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:49:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:49:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:49:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:49:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773807/SRX9773807.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling