Job ID = 14522039 SRX = SRX9773806 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 38053700 spots for SRR13347747/SRR13347747.sra Written 38053700 spots for SRR13347747/SRR13347747.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:28 38053700 reads; of these: 38053700 (100.00%) were paired; of these: 2050068 (5.39%) aligned concordantly 0 times 29512613 (77.56%) aligned concordantly exactly 1 time 6491019 (17.06%) aligned concordantly >1 times ---- 2050068 pairs aligned concordantly 0 times; of these: 744906 (36.34%) aligned discordantly 1 time ---- 1305162 pairs aligned 0 times concordantly or discordantly; of these: 2610324 mates make up the pairs; of these: 1621057 (62.10%) aligned 0 times 405749 (15.54%) aligned exactly 1 time 583518 (22.35%) aligned >1 times 97.87% overall alignment rate Time searching: 00:31:28 Overall time: 00:31:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 19454428 / 36696043 = 0.5302 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:51:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:51:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:51:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:51:15: 1000000 INFO @ Sat, 15 Jan 2022 22:51:22: 2000000 INFO @ Sat, 15 Jan 2022 22:51:28: 3000000 INFO @ Sat, 15 Jan 2022 22:51:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:51:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:51:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:51:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:51:42: 5000000 INFO @ Sat, 15 Jan 2022 22:51:45: 1000000 INFO @ Sat, 15 Jan 2022 22:51:48: 6000000 INFO @ Sat, 15 Jan 2022 22:51:52: 2000000 INFO @ Sat, 15 Jan 2022 22:51:55: 7000000 INFO @ Sat, 15 Jan 2022 22:51:59: 3000000 INFO @ Sat, 15 Jan 2022 22:52:02: 8000000 INFO @ Sat, 15 Jan 2022 22:52:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:52:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:52:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:52:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:52:09: 9000000 INFO @ Sat, 15 Jan 2022 22:52:12: 5000000 INFO @ Sat, 15 Jan 2022 22:52:15: 1000000 INFO @ Sat, 15 Jan 2022 22:52:16: 10000000 INFO @ Sat, 15 Jan 2022 22:52:19: 6000000 INFO @ Sat, 15 Jan 2022 22:52:22: 2000000 INFO @ Sat, 15 Jan 2022 22:52:22: 11000000 INFO @ Sat, 15 Jan 2022 22:52:26: 7000000 INFO @ Sat, 15 Jan 2022 22:52:29: 3000000 INFO @ Sat, 15 Jan 2022 22:52:29: 12000000 INFO @ Sat, 15 Jan 2022 22:52:33: 8000000 INFO @ Sat, 15 Jan 2022 22:52:36: 4000000 INFO @ Sat, 15 Jan 2022 22:52:36: 13000000 INFO @ Sat, 15 Jan 2022 22:52:40: 9000000 INFO @ Sat, 15 Jan 2022 22:52:43: 5000000 INFO @ Sat, 15 Jan 2022 22:52:43: 14000000 INFO @ Sat, 15 Jan 2022 22:52:47: 10000000 INFO @ Sat, 15 Jan 2022 22:52:49: 6000000 INFO @ Sat, 15 Jan 2022 22:52:50: 15000000 INFO @ Sat, 15 Jan 2022 22:52:53: 11000000 INFO @ Sat, 15 Jan 2022 22:52:56: 7000000 INFO @ Sat, 15 Jan 2022 22:52:57: 16000000 INFO @ Sat, 15 Jan 2022 22:53:00: 12000000 INFO @ Sat, 15 Jan 2022 22:53:03: 8000000 INFO @ Sat, 15 Jan 2022 22:53:04: 17000000 INFO @ Sat, 15 Jan 2022 22:53:07: 13000000 INFO @ Sat, 15 Jan 2022 22:53:10: 9000000 INFO @ Sat, 15 Jan 2022 22:53:10: 18000000 INFO @ Sat, 15 Jan 2022 22:53:14: 14000000 INFO @ Sat, 15 Jan 2022 22:53:17: 10000000 INFO @ Sat, 15 Jan 2022 22:53:17: 19000000 INFO @ Sat, 15 Jan 2022 22:53:21: 15000000 INFO @ Sat, 15 Jan 2022 22:53:24: 11000000 INFO @ Sat, 15 Jan 2022 22:53:24: 20000000 INFO @ Sat, 15 Jan 2022 22:53:28: 16000000 INFO @ Sat, 15 Jan 2022 22:53:30: 12000000 INFO @ Sat, 15 Jan 2022 22:53:31: 21000000 INFO @ Sat, 15 Jan 2022 22:53:34: 17000000 INFO @ Sat, 15 Jan 2022 22:53:37: 13000000 INFO @ Sat, 15 Jan 2022 22:53:37: 22000000 INFO @ Sat, 15 Jan 2022 22:53:41: 18000000 INFO @ Sat, 15 Jan 2022 22:53:44: 23000000 INFO @ Sat, 15 Jan 2022 22:53:44: 14000000 INFO @ Sat, 15 Jan 2022 22:53:48: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:53:51: 24000000 INFO @ Sat, 15 Jan 2022 22:53:51: 15000000 INFO @ Sat, 15 Jan 2022 22:53:55: 20000000 INFO @ Sat, 15 Jan 2022 22:53:57: 25000000 INFO @ Sat, 15 Jan 2022 22:53:58: 16000000 INFO @ Sat, 15 Jan 2022 22:54:02: 21000000 INFO @ Sat, 15 Jan 2022 22:54:04: 26000000 INFO @ Sat, 15 Jan 2022 22:54:05: 17000000 INFO @ Sat, 15 Jan 2022 22:54:08: 22000000 INFO @ Sat, 15 Jan 2022 22:54:11: 27000000 INFO @ Sat, 15 Jan 2022 22:54:11: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:54:15: 23000000 INFO @ Sat, 15 Jan 2022 22:54:18: 28000000 INFO @ Sat, 15 Jan 2022 22:54:18: 19000000 INFO @ Sat, 15 Jan 2022 22:54:22: 24000000 INFO @ Sat, 15 Jan 2022 22:54:25: 29000000 INFO @ Sat, 15 Jan 2022 22:54:25: 20000000 INFO @ Sat, 15 Jan 2022 22:54:29: 25000000 INFO @ Sat, 15 Jan 2022 22:54:32: 30000000 INFO @ Sat, 15 Jan 2022 22:54:32: 21000000 INFO @ Sat, 15 Jan 2022 22:54:36: 26000000 INFO @ Sat, 15 Jan 2022 22:54:38: 31000000 INFO @ Sat, 15 Jan 2022 22:54:39: 22000000 INFO @ Sat, 15 Jan 2022 22:54:43: 27000000 INFO @ Sat, 15 Jan 2022 22:54:45: 32000000 INFO @ Sat, 15 Jan 2022 22:54:46: 23000000 INFO @ Sat, 15 Jan 2022 22:54:49: 28000000 INFO @ Sat, 15 Jan 2022 22:54:52: 33000000 INFO @ Sat, 15 Jan 2022 22:54:53: 24000000 INFO @ Sat, 15 Jan 2022 22:54:56: 29000000 INFO @ Sat, 15 Jan 2022 22:54:59: 34000000 INFO @ Sat, 15 Jan 2022 22:54:59: 25000000 INFO @ Sat, 15 Jan 2022 22:55:03: 30000000 INFO @ Sat, 15 Jan 2022 22:55:06: 35000000 INFO @ Sat, 15 Jan 2022 22:55:06: 26000000 INFO @ Sat, 15 Jan 2022 22:55:10: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:55:10: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:55:10: #1 total tags in treatment: 16772338 INFO @ Sat, 15 Jan 2022 22:55:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:55:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:55:10: 31000000 INFO @ Sat, 15 Jan 2022 22:55:10: #1 tags after filtering in treatment: 9939750 INFO @ Sat, 15 Jan 2022 22:55:10: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:55:10: #1 finished! INFO @ Sat, 15 Jan 2022 22:55:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:55:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:55:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:55:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:55:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:55:13: 27000000 INFO @ Sat, 15 Jan 2022 22:55:17: 32000000 INFO @ Sat, 15 Jan 2022 22:55:20: 28000000 INFO @ Sat, 15 Jan 2022 22:55:23: 33000000 INFO @ Sat, 15 Jan 2022 22:55:27: 29000000 INFO @ Sat, 15 Jan 2022 22:55:30: 34000000 INFO @ Sat, 15 Jan 2022 22:55:33: 30000000 INFO @ Sat, 15 Jan 2022 22:55:37: 35000000 INFO @ Sat, 15 Jan 2022 22:55:40: 31000000 INFO @ Sat, 15 Jan 2022 22:55:41: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:55:41: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:55:41: #1 total tags in treatment: 16772338 INFO @ Sat, 15 Jan 2022 22:55:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:55:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:55:41: #1 tags after filtering in treatment: 9939750 INFO @ Sat, 15 Jan 2022 22:55:41: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:55:41: #1 finished! INFO @ Sat, 15 Jan 2022 22:55:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:55:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:55:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:55:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:55:47: 32000000 INFO @ Sat, 15 Jan 2022 22:55:53: 33000000 INFO @ Sat, 15 Jan 2022 22:56:00: 34000000 INFO @ Sat, 15 Jan 2022 22:56:07: 35000000 INFO @ Sat, 15 Jan 2022 22:56:10: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:56:10: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:56:10: #1 total tags in treatment: 16772338 INFO @ Sat, 15 Jan 2022 22:56:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:56:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:56:11: #1 tags after filtering in treatment: 9939750 INFO @ Sat, 15 Jan 2022 22:56:11: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 22:56:11: #1 finished! INFO @ Sat, 15 Jan 2022 22:56:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:56:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:56:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:56:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:56:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9773806/SRX9773806.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling