Job ID = 9163636 sra ファイルのダウンロード中... Completed: 468966K bytes transferred in 7 seconds (503340K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 7524974 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974684/SRR1948326.sra Written 7524974 spots total Written 7686348 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974684/SRR1948327.sra Written 7686348 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:20 15211322 reads; of these: 15211322 (100.00%) were unpaired; of these: 410603 (2.70%) aligned 0 times 12846015 (84.45%) aligned exactly 1 time 1954704 (12.85%) aligned >1 times 97.30% overall alignment rate Time searching: 00:04:20 Overall time: 00:04:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5649287 / 14800719 = 0.3817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:17:50: # Command line: callpeak -t SRX974684.bam -f BAM -g 12100000 -n SRX974684.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX974684.10 # format = BAM # ChIP-seq file = ['SRX974684.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:17:50: # Command line: callpeak -t SRX974684.bam -f BAM -g 12100000 -n SRX974684.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX974684.20 # format = BAM # ChIP-seq file = ['SRX974684.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:17:50: # Command line: callpeak -t SRX974684.bam -f BAM -g 12100000 -n SRX974684.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX974684.05 # format = BAM # ChIP-seq file = ['SRX974684.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:17:50: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:17:50: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:17:57: 1000000 INFO @ Wed, 28 Jun 2017 11:17:57: 1000000 INFO @ Wed, 28 Jun 2017 11:17:57: 1000000 INFO @ Wed, 28 Jun 2017 11:18:04: 2000000 INFO @ Wed, 28 Jun 2017 11:18:04: 2000000 INFO @ Wed, 28 Jun 2017 11:18:04: 2000000 INFO @ Wed, 28 Jun 2017 11:18:11: 3000000 INFO @ Wed, 28 Jun 2017 11:18:11: 3000000 INFO @ Wed, 28 Jun 2017 11:18:12: 3000000 INFO @ Wed, 28 Jun 2017 11:18:17: 4000000 INFO @ Wed, 28 Jun 2017 11:18:18: 4000000 INFO @ Wed, 28 Jun 2017 11:18:19: 4000000 INFO @ Wed, 28 Jun 2017 11:18:24: 5000000 INFO @ Wed, 28 Jun 2017 11:18:25: 5000000 INFO @ Wed, 28 Jun 2017 11:18:26: 5000000 INFO @ Wed, 28 Jun 2017 11:18:31: 6000000 INFO @ Wed, 28 Jun 2017 11:18:32: 6000000 INFO @ Wed, 28 Jun 2017 11:18:33: 6000000 INFO @ Wed, 28 Jun 2017 11:18:38: 7000000 INFO @ Wed, 28 Jun 2017 11:18:38: 7000000 INFO @ Wed, 28 Jun 2017 11:18:40: 7000000 INFO @ Wed, 28 Jun 2017 11:18:45: 8000000 INFO @ Wed, 28 Jun 2017 11:18:45: 8000000 INFO @ Wed, 28 Jun 2017 11:18:48: 8000000 INFO @ Wed, 28 Jun 2017 11:18:51: 9000000 INFO @ Wed, 28 Jun 2017 11:18:52: 9000000 INFO @ Wed, 28 Jun 2017 11:18:52: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:18:52: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:18:52: #1 total tags in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:52: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:18:53: #1 tags after filtering in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:53: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:53: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:53: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:53: Process for pairing-model is terminated! cat: SRX974684.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 11:18:53: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:18:53: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:18:53: #1 total tags in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:53: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974684.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:18:53: #1 tags after filtering in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:53: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:53: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:54: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:54: Process for pairing-model is terminated! cat: SRX974684.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974684.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:18:55: 9000000 INFO @ Wed, 28 Jun 2017 11:18:56: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:18:56: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:18:56: #1 total tags in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:56: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:18:56: #1 tags after filtering in treatment: 9151432 INFO @ Wed, 28 Jun 2017 11:18:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:56: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:56: Process for pairing-model is terminated! cat: SRX974684.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974684.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974684.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。