Job ID = 9163627 sra ファイルのダウンロード中... Completed: 486611K bytes transferred in 8 seconds (491386K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 7833396 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974676/SRR1948310.sra Written 7833396 spots total Written 8009228 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974676/SRR1948311.sra Written 8009228 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:11 15842624 reads; of these: 15842624 (100.00%) were unpaired; of these: 289429 (1.83%) aligned 0 times 13343010 (84.22%) aligned exactly 1 time 2210185 (13.95%) aligned >1 times 98.17% overall alignment rate Time searching: 00:03:11 Overall time: 00:03:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6972897 / 15553195 = 0.4483 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:15:25: # Command line: callpeak -t SRX974676.bam -f BAM -g 12100000 -n SRX974676.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX974676.10 # format = BAM # ChIP-seq file = ['SRX974676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:25: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:25: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:25: # Command line: callpeak -t SRX974676.bam -f BAM -g 12100000 -n SRX974676.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX974676.20 # format = BAM # ChIP-seq file = ['SRX974676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:25: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:25: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:25: # Command line: callpeak -t SRX974676.bam -f BAM -g 12100000 -n SRX974676.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX974676.05 # format = BAM # ChIP-seq file = ['SRX974676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:25: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:25: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:32: 1000000 INFO @ Wed, 28 Jun 2017 11:15:32: 1000000 INFO @ Wed, 28 Jun 2017 11:15:33: 1000000 INFO @ Wed, 28 Jun 2017 11:15:38: 2000000 INFO @ Wed, 28 Jun 2017 11:15:39: 2000000 INFO @ Wed, 28 Jun 2017 11:15:41: 2000000 INFO @ Wed, 28 Jun 2017 11:15:45: 3000000 INFO @ Wed, 28 Jun 2017 11:15:45: 3000000 INFO @ Wed, 28 Jun 2017 11:15:50: 3000000 INFO @ Wed, 28 Jun 2017 11:15:51: 4000000 INFO @ Wed, 28 Jun 2017 11:15:52: 4000000 INFO @ Wed, 28 Jun 2017 11:15:58: 4000000 INFO @ Wed, 28 Jun 2017 11:15:58: 5000000 INFO @ Wed, 28 Jun 2017 11:16:00: 5000000 INFO @ Wed, 28 Jun 2017 11:16:05: 6000000 INFO @ Wed, 28 Jun 2017 11:16:06: 5000000 INFO @ Wed, 28 Jun 2017 11:16:07: 6000000 INFO @ Wed, 28 Jun 2017 11:16:12: 7000000 INFO @ Wed, 28 Jun 2017 11:16:14: 7000000 INFO @ Wed, 28 Jun 2017 11:16:15: 6000000 INFO @ Wed, 28 Jun 2017 11:16:19: 8000000 INFO @ Wed, 28 Jun 2017 11:16:21: 8000000 INFO @ Wed, 28 Jun 2017 11:16:22: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:16:22: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:16:22: #1 total tags in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:22: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:23: #1 tags after filtering in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:23: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:23: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:23: 7000000 INFO @ Wed, 28 Jun 2017 11:16:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:23: Process for pairing-model is terminated! cat: SRX974676.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974676.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:16:24: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:16:24: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:16:24: #1 total tags in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:24: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:25: #1 tags after filtering in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:25: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:25: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:25: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:25: Process for pairing-model is terminated! cat: SRX974676.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974676.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:16:31: 8000000 INFO @ Wed, 28 Jun 2017 11:16:35: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:16:35: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:16:35: #1 total tags in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:35: #1 tags after filtering in treatment: 8580298 INFO @ Wed, 28 Jun 2017 11:16:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:35: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:35: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:36: Process for pairing-model is terminated! cat: SRX974676.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974676.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974676.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。