Job ID = 9163623 sra ファイルのダウンロード中... Completed: 520988K bytes transferred in 7 seconds (540303K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 8359081 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974672/SRR1948302.sra Written 8359081 spots total Written 8510029 spots for /home/okishinya/chipatlas/results/sacCer3/SRX974672/SRR1948303.sra Written 8510029 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:45 16869110 reads; of these: 16869110 (100.00%) were unpaired; of these: 382153 (2.27%) aligned 0 times 13889737 (82.34%) aligned exactly 1 time 2597220 (15.40%) aligned >1 times 97.73% overall alignment rate Time searching: 00:03:45 Overall time: 00:03:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7919635 / 16486957 = 0.4804 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:14:39: # Command line: callpeak -t SRX974672.bam -f BAM -g 12100000 -n SRX974672.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX974672.20 # format = BAM # ChIP-seq file = ['SRX974672.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:39: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:39: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:39: # Command line: callpeak -t SRX974672.bam -f BAM -g 12100000 -n SRX974672.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX974672.10 # format = BAM # ChIP-seq file = ['SRX974672.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:39: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:39: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:39: # Command line: callpeak -t SRX974672.bam -f BAM -g 12100000 -n SRX974672.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX974672.05 # format = BAM # ChIP-seq file = ['SRX974672.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:39: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:39: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:47: 1000000 INFO @ Wed, 28 Jun 2017 11:14:47: 1000000 INFO @ Wed, 28 Jun 2017 11:14:47: 1000000 INFO @ Wed, 28 Jun 2017 11:14:54: 2000000 INFO @ Wed, 28 Jun 2017 11:14:54: 2000000 INFO @ Wed, 28 Jun 2017 11:14:55: 2000000 INFO @ Wed, 28 Jun 2017 11:15:02: 3000000 INFO @ Wed, 28 Jun 2017 11:15:02: 3000000 INFO @ Wed, 28 Jun 2017 11:15:04: 3000000 INFO @ Wed, 28 Jun 2017 11:15:10: 4000000 INFO @ Wed, 28 Jun 2017 11:15:10: 4000000 INFO @ Wed, 28 Jun 2017 11:15:13: 4000000 INFO @ Wed, 28 Jun 2017 11:15:18: 5000000 INFO @ Wed, 28 Jun 2017 11:15:18: 5000000 INFO @ Wed, 28 Jun 2017 11:15:22: 5000000 INFO @ Wed, 28 Jun 2017 11:15:27: 6000000 INFO @ Wed, 28 Jun 2017 11:15:27: 6000000 INFO @ Wed, 28 Jun 2017 11:15:31: 6000000 INFO @ Wed, 28 Jun 2017 11:15:34: 7000000 INFO @ Wed, 28 Jun 2017 11:15:34: 7000000 INFO @ Wed, 28 Jun 2017 11:15:40: 7000000 INFO @ Wed, 28 Jun 2017 11:15:42: 8000000 INFO @ Wed, 28 Jun 2017 11:15:42: 8000000 INFO @ Wed, 28 Jun 2017 11:15:46: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:15:46: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:15:46: #1 total tags in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:46: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:46: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:15:46: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:15:46: #1 total tags in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:46: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:46: #1 tags after filtering in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:46: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:46: #1 tags after filtering in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:46: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:46: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:46: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 11:15:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:46: Process for pairing-model is terminated! cat: SRX974672.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX974672.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974672.20_model.r'rm: cannot remove `SRX974672.10_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:15:47: 8000000 INFO @ Wed, 28 Jun 2017 11:15:50: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 11:15:50: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 11:15:50: #1 total tags in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:50: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:51: #1 tags after filtering in treatment: 8567322 INFO @ Wed, 28 Jun 2017 11:15:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:51: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:51: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:51: Process for pairing-model is terminated! cat: SRX974672.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX974672.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX974672.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。