Job ID = 9163615 sra ファイルのダウンロード中... Completed: 284324K bytes transferred in 5 seconds (415504K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 14424608 spots for /home/okishinya/chipatlas/results/sacCer3/SRX966744/SRR1927160.sra Written 14424608 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:45 14424608 reads; of these: 14424608 (100.00%) were unpaired; of these: 1493733 (10.36%) aligned 0 times 11278037 (78.19%) aligned exactly 1 time 1652838 (11.46%) aligned >1 times 89.64% overall alignment rate Time searching: 00:03:45 Overall time: 00:03:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4306349 / 12930875 = 0.3330 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:13:22: # Command line: callpeak -t SRX966744.bam -f BAM -g 12100000 -n SRX966744.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX966744.20 # format = BAM # ChIP-seq file = ['SRX966744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:22: # Command line: callpeak -t SRX966744.bam -f BAM -g 12100000 -n SRX966744.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX966744.10 # format = BAM # ChIP-seq file = ['SRX966744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:22: # Command line: callpeak -t SRX966744.bam -f BAM -g 12100000 -n SRX966744.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX966744.05 # format = BAM # ChIP-seq file = ['SRX966744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:22: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:22: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:32: 1000000 INFO @ Wed, 28 Jun 2017 11:13:33: 1000000 INFO @ Wed, 28 Jun 2017 11:13:33: 1000000 INFO @ Wed, 28 Jun 2017 11:13:43: 2000000 INFO @ Wed, 28 Jun 2017 11:13:43: 2000000 INFO @ Wed, 28 Jun 2017 11:13:44: 2000000 INFO @ Wed, 28 Jun 2017 11:13:54: 3000000 INFO @ Wed, 28 Jun 2017 11:13:55: 3000000 INFO @ Wed, 28 Jun 2017 11:13:55: 3000000 INFO @ Wed, 28 Jun 2017 11:14:05: 4000000 INFO @ Wed, 28 Jun 2017 11:14:07: 4000000 INFO @ Wed, 28 Jun 2017 11:14:08: 4000000 INFO @ Wed, 28 Jun 2017 11:14:15: 5000000 INFO @ Wed, 28 Jun 2017 11:14:17: 5000000 INFO @ Wed, 28 Jun 2017 11:14:20: 5000000 INFO @ Wed, 28 Jun 2017 11:14:26: 6000000 INFO @ Wed, 28 Jun 2017 11:14:29: 6000000 INFO @ Wed, 28 Jun 2017 11:14:33: 6000000 INFO @ Wed, 28 Jun 2017 11:14:38: 7000000 INFO @ Wed, 28 Jun 2017 11:14:40: 7000000 INFO @ Wed, 28 Jun 2017 11:14:46: 7000000 INFO @ Wed, 28 Jun 2017 11:14:49: 8000000 INFO @ Wed, 28 Jun 2017 11:14:51: 8000000 INFO @ Wed, 28 Jun 2017 11:14:55: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:14:55: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:14:55: #1 total tags in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:14:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:55: #1 tags after filtering in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:14:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:55: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:55: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:55: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:55: Process for pairing-model is terminated! cat: SRX966744.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX966744.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:14:57: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:14:57: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:14:57: #1 total tags in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:14:57: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:58: #1 tags after filtering in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:14:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:58: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:58: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:58: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:58: Process for pairing-model is terminated! cat: SRX966744.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX966744.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:14:58: 8000000 INFO @ Wed, 28 Jun 2017 11:15:06: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:15:06: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:15:06: #1 total tags in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:15:06: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:06: #1 tags after filtering in treatment: 8624526 INFO @ Wed, 28 Jun 2017 11:15:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:06: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:06: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:06: Process for pairing-model is terminated! cat: SRX966744.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX966744.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX966744.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。