Job ID = 9163609 sra ファイルのダウンロード中... Completed: 722628K bytes transferred in 10 seconds (566743K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21745692 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964232/SRR1924339.sra Written 21745692 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:06 21745692 reads; of these: 21745692 (100.00%) were unpaired; of these: 886812 (4.08%) aligned 0 times 15293682 (70.33%) aligned exactly 1 time 5565198 (25.59%) aligned >1 times 95.92% overall alignment rate Time searching: 00:04:06 Overall time: 00:04:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9502897 / 20858880 = 0.4556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:14:19: # Command line: callpeak -t SRX964232.bam -f BAM -g 12100000 -n SRX964232.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964232.10 # format = BAM # ChIP-seq file = ['SRX964232.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:19: # Command line: callpeak -t SRX964232.bam -f BAM -g 12100000 -n SRX964232.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964232.20 # format = BAM # ChIP-seq file = ['SRX964232.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:19: # Command line: callpeak -t SRX964232.bam -f BAM -g 12100000 -n SRX964232.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964232.05 # format = BAM # ChIP-seq file = ['SRX964232.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:19: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:19: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:26: 1000000 INFO @ Wed, 28 Jun 2017 11:14:26: 1000000 INFO @ Wed, 28 Jun 2017 11:14:26: 1000000 INFO @ Wed, 28 Jun 2017 11:14:33: 2000000 INFO @ Wed, 28 Jun 2017 11:14:33: 2000000 INFO @ Wed, 28 Jun 2017 11:14:33: 2000000 INFO @ Wed, 28 Jun 2017 11:14:40: 3000000 INFO @ Wed, 28 Jun 2017 11:14:41: 3000000 INFO @ Wed, 28 Jun 2017 11:14:41: 3000000 INFO @ Wed, 28 Jun 2017 11:14:48: 4000000 INFO @ Wed, 28 Jun 2017 11:14:48: 4000000 INFO @ Wed, 28 Jun 2017 11:14:48: 4000000 INFO @ Wed, 28 Jun 2017 11:14:55: 5000000 INFO @ Wed, 28 Jun 2017 11:14:55: 5000000 INFO @ Wed, 28 Jun 2017 11:14:56: 5000000 INFO @ Wed, 28 Jun 2017 11:15:01: 6000000 INFO @ Wed, 28 Jun 2017 11:15:01: 6000000 INFO @ Wed, 28 Jun 2017 11:15:04: 6000000 INFO @ Wed, 28 Jun 2017 11:15:08: 7000000 INFO @ Wed, 28 Jun 2017 11:15:08: 7000000 INFO @ Wed, 28 Jun 2017 11:15:12: 7000000 INFO @ Wed, 28 Jun 2017 11:15:15: 8000000 INFO @ Wed, 28 Jun 2017 11:15:15: 8000000 INFO @ Wed, 28 Jun 2017 11:15:20: 8000000 INFO @ Wed, 28 Jun 2017 11:15:21: 9000000 INFO @ Wed, 28 Jun 2017 11:15:21: 9000000 INFO @ Wed, 28 Jun 2017 11:15:28: 10000000 INFO @ Wed, 28 Jun 2017 11:15:28: 10000000 INFO @ Wed, 28 Jun 2017 11:15:29: 9000000 INFO @ Wed, 28 Jun 2017 11:15:34: 11000000 INFO @ Wed, 28 Jun 2017 11:15:34: 11000000 INFO @ Wed, 28 Jun 2017 11:15:36: 10000000 INFO @ Wed, 28 Jun 2017 11:15:37: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:37: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:37: #1 total tags in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:37: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:37: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:37: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:37: #1 total tags in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:37: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:37: #1 tags after filtering in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:37: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:37: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:37: #1 tags after filtering in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:37: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:37: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:38: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:38: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 11:15:38: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:38: Process for pairing-model is terminated! cat: SRX964232.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX964232.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964232.20_model.r'rm: cannot remove `SRX964232.10_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:15:43: 11000000 INFO @ Wed, 28 Jun 2017 11:15:45: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:45: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:45: #1 total tags in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:45: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:45: #1 tags after filtering in treatment: 11355983 INFO @ Wed, 28 Jun 2017 11:15:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:45: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:45: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:46: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:46: Process for pairing-model is terminated! cat: SRX964232.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964232.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964232.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。