Job ID = 9163608 sra ファイルのダウンロード中... Completed: 744705K bytes transferred in 11 seconds (527579K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25778764 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964231/SRR1924338.sra Written 25778764 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:24 25778764 reads; of these: 25778764 (100.00%) were unpaired; of these: 2614039 (10.14%) aligned 0 times 20156407 (78.19%) aligned exactly 1 time 3008318 (11.67%) aligned >1 times 89.86% overall alignment rate Time searching: 00:04:24 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11122617 / 23164725 = 0.4802 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:14:54: # Command line: callpeak -t SRX964231.bam -f BAM -g 12100000 -n SRX964231.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964231.20 # format = BAM # ChIP-seq file = ['SRX964231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:54: # Command line: callpeak -t SRX964231.bam -f BAM -g 12100000 -n SRX964231.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964231.10 # format = BAM # ChIP-seq file = ['SRX964231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:54: # Command line: callpeak -t SRX964231.bam -f BAM -g 12100000 -n SRX964231.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964231.05 # format = BAM # ChIP-seq file = ['SRX964231.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:54: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:54: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:02: 1000000 INFO @ Wed, 28 Jun 2017 11:15:02: 1000000 INFO @ Wed, 28 Jun 2017 11:15:02: 1000000 INFO @ Wed, 28 Jun 2017 11:15:09: 2000000 INFO @ Wed, 28 Jun 2017 11:15:09: 2000000 INFO @ Wed, 28 Jun 2017 11:15:09: 2000000 INFO @ Wed, 28 Jun 2017 11:15:17: 3000000 INFO @ Wed, 28 Jun 2017 11:15:17: 3000000 INFO @ Wed, 28 Jun 2017 11:15:17: 3000000 INFO @ Wed, 28 Jun 2017 11:15:24: 4000000 INFO @ Wed, 28 Jun 2017 11:15:24: 4000000 INFO @ Wed, 28 Jun 2017 11:15:26: 4000000 INFO @ Wed, 28 Jun 2017 11:15:32: 5000000 INFO @ Wed, 28 Jun 2017 11:15:32: 5000000 INFO @ Wed, 28 Jun 2017 11:15:35: 5000000 INFO @ Wed, 28 Jun 2017 11:15:41: 6000000 INFO @ Wed, 28 Jun 2017 11:15:41: 6000000 INFO @ Wed, 28 Jun 2017 11:15:44: 6000000 INFO @ Wed, 28 Jun 2017 11:15:49: 7000000 INFO @ Wed, 28 Jun 2017 11:15:49: 7000000 INFO @ Wed, 28 Jun 2017 11:15:53: 7000000 INFO @ Wed, 28 Jun 2017 11:15:57: 8000000 INFO @ Wed, 28 Jun 2017 11:15:57: 8000000 INFO @ Wed, 28 Jun 2017 11:16:01: 8000000 INFO @ Wed, 28 Jun 2017 11:16:06: 9000000 INFO @ Wed, 28 Jun 2017 11:16:06: 9000000 INFO @ Wed, 28 Jun 2017 11:16:09: 9000000 INFO @ Wed, 28 Jun 2017 11:16:13: 10000000 INFO @ Wed, 28 Jun 2017 11:16:13: 10000000 INFO @ Wed, 28 Jun 2017 11:16:16: 10000000 INFO @ Wed, 28 Jun 2017 11:16:21: 11000000 INFO @ Wed, 28 Jun 2017 11:16:21: 11000000 INFO @ Wed, 28 Jun 2017 11:16:24: 11000000 INFO @ Wed, 28 Jun 2017 11:16:28: 12000000 INFO @ Wed, 28 Jun 2017 11:16:28: 12000000 INFO @ Wed, 28 Jun 2017 11:16:29: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:16:29: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:16:29: #1 total tags in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:29: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:29: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:16:29: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:16:29: #1 total tags in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:29: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:29: #1 tags after filtering in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:29: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:29: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:29: #1 tags after filtering in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:29: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:29: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:30: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:30: Process for pairing-model is terminated! cat: SRX964231.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis INFO @ Wed, 28 Jun 2017 11:16:30: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:30: Process for pairing-model is terminated! needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964231.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX964231.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964231.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:16:31: 12000000 INFO @ Wed, 28 Jun 2017 11:16:32: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:16:32: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:16:32: #1 total tags in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:32: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:16:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:16:32: #1 tags after filtering in treatment: 12042108 INFO @ Wed, 28 Jun 2017 11:16:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:16:32: #1 finished! INFO @ Wed, 28 Jun 2017 11:16:32: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:16:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:16:33: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:16:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:16:33: Process for pairing-model is terminated! cat: SRX964231.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964231.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964231.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。