Job ID = 9163602 sra ファイルのダウンロード中... Completed: 949033K bytes transferred in 10 seconds (724661K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28792520 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964225/SRR1924332.sra Written 28792520 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:00 28792520 reads; of these: 28792520 (100.00%) were unpaired; of these: 3591705 (12.47%) aligned 0 times 20849795 (72.41%) aligned exactly 1 time 4351020 (15.11%) aligned >1 times 87.53% overall alignment rate Time searching: 00:06:00 Overall time: 00:06:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13114234 / 25200815 = 0.5204 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:15:38: # Command line: callpeak -t SRX964225.bam -f BAM -g 12100000 -n SRX964225.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964225.10 # format = BAM # ChIP-seq file = ['SRX964225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:38: # Command line: callpeak -t SRX964225.bam -f BAM -g 12100000 -n SRX964225.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964225.20 # format = BAM # ChIP-seq file = ['SRX964225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:38: # Command line: callpeak -t SRX964225.bam -f BAM -g 12100000 -n SRX964225.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964225.05 # format = BAM # ChIP-seq file = ['SRX964225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:15:38: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:15:38: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:15:45: 1000000 INFO @ Wed, 28 Jun 2017 11:15:45: 1000000 INFO @ Wed, 28 Jun 2017 11:15:45: 1000000 INFO @ Wed, 28 Jun 2017 11:15:52: 2000000 INFO @ Wed, 28 Jun 2017 11:15:52: 2000000 INFO @ Wed, 28 Jun 2017 11:15:52: 2000000 INFO @ Wed, 28 Jun 2017 11:15:59: 3000000 INFO @ Wed, 28 Jun 2017 11:15:59: 3000000 INFO @ Wed, 28 Jun 2017 11:16:00: 3000000 INFO @ Wed, 28 Jun 2017 11:16:06: 4000000 INFO @ Wed, 28 Jun 2017 11:16:06: 4000000 INFO @ Wed, 28 Jun 2017 11:16:08: 4000000 INFO @ Wed, 28 Jun 2017 11:16:13: 5000000 INFO @ Wed, 28 Jun 2017 11:16:13: 5000000 INFO @ Wed, 28 Jun 2017 11:16:15: 5000000 INFO @ Wed, 28 Jun 2017 11:16:19: 6000000 INFO @ Wed, 28 Jun 2017 11:16:20: 6000000 INFO @ Wed, 28 Jun 2017 11:16:23: 6000000 INFO @ Wed, 28 Jun 2017 11:16:26: 7000000 INFO @ Wed, 28 Jun 2017 11:16:27: 7000000 INFO @ Wed, 28 Jun 2017 11:16:30: 7000000 INFO @ Wed, 28 Jun 2017 11:16:33: 8000000 INFO @ Wed, 28 Jun 2017 11:16:34: 8000000 INFO @ Wed, 28 Jun 2017 11:16:38: 8000000 INFO @ Wed, 28 Jun 2017 11:16:40: 9000000 INFO @ Wed, 28 Jun 2017 11:16:41: 9000000 INFO @ Wed, 28 Jun 2017 11:16:46: 9000000 INFO @ Wed, 28 Jun 2017 11:16:47: 10000000 INFO @ Wed, 28 Jun 2017 11:16:48: 10000000 INFO @ Wed, 28 Jun 2017 11:16:53: 10000000 INFO @ Wed, 28 Jun 2017 11:16:54: 11000000 INFO @ Wed, 28 Jun 2017 11:16:55: 11000000 INFO @ Wed, 28 Jun 2017 11:17:01: 11000000 INFO @ Wed, 28 Jun 2017 11:17:01: 12000000 INFO @ Wed, 28 Jun 2017 11:17:02: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:17:02: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:17:02: #1 total tags in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:02: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:17:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:17:02: #1 tags after filtering in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:17:02: #1 finished! INFO @ Wed, 28 Jun 2017 11:17:02: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:17:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:17:02: 12000000 INFO @ Wed, 28 Jun 2017 11:17:03: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:17:03: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:17:03: #1 total tags in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:03: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:17:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:17:03: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:17:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:17:03: Process for pairing-model is terminated! cat: SRX964225.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964225.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:17:03: #1 tags after filtering in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:17:03: #1 finished! INFO @ Wed, 28 Jun 2017 11:17:03: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:17:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:17:03: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:17:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:17:03: Process for pairing-model is terminated! cat: SRX964225.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964225.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:17:07: 12000000 INFO @ Wed, 28 Jun 2017 11:17:08: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:17:08: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:17:08: #1 total tags in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:08: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:17:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:17:08: #1 tags after filtering in treatment: 12086581 INFO @ Wed, 28 Jun 2017 11:17:08: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:17:08: #1 finished! INFO @ Wed, 28 Jun 2017 11:17:08: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:17:08: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:17:09: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:17:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:17:09: Process for pairing-model is terminated! cat: SRX964225.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964225.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964225.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。