Job ID = 9163599 sra ファイルのダウンロード中... Completed: 650304K bytes transferred in 10 seconds (495098K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19582861 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964222/SRR1924329.sra Written 19582861 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:44 19582861 reads; of these: 19582861 (100.00%) were unpaired; of these: 818475 (4.18%) aligned 0 times 14521192 (74.15%) aligned exactly 1 time 4243194 (21.67%) aligned >1 times 95.82% overall alignment rate Time searching: 00:03:44 Overall time: 00:03:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7748644 / 18764386 = 0.4129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:11:08: # Command line: callpeak -t SRX964222.bam -f BAM -g 12100000 -n SRX964222.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964222.10 # format = BAM # ChIP-seq file = ['SRX964222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:08: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:08: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:08: # Command line: callpeak -t SRX964222.bam -f BAM -g 12100000 -n SRX964222.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964222.20 # format = BAM # ChIP-seq file = ['SRX964222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:08: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:08: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:08: # Command line: callpeak -t SRX964222.bam -f BAM -g 12100000 -n SRX964222.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964222.05 # format = BAM # ChIP-seq file = ['SRX964222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:08: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:08: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:16: 1000000 INFO @ Wed, 28 Jun 2017 11:11:16: 1000000 INFO @ Wed, 28 Jun 2017 11:11:16: 1000000 INFO @ Wed, 28 Jun 2017 11:11:24: 2000000 INFO @ Wed, 28 Jun 2017 11:11:24: 2000000 INFO @ Wed, 28 Jun 2017 11:11:25: 2000000 INFO @ Wed, 28 Jun 2017 11:11:31: 3000000 INFO @ Wed, 28 Jun 2017 11:11:32: 3000000 INFO @ Wed, 28 Jun 2017 11:11:33: 3000000 INFO @ Wed, 28 Jun 2017 11:11:37: 4000000 INFO @ Wed, 28 Jun 2017 11:11:39: 4000000 INFO @ Wed, 28 Jun 2017 11:11:40: 4000000 INFO @ Wed, 28 Jun 2017 11:11:44: 5000000 INFO @ Wed, 28 Jun 2017 11:11:46: 5000000 INFO @ Wed, 28 Jun 2017 11:11:48: 5000000 INFO @ Wed, 28 Jun 2017 11:11:50: 6000000 INFO @ Wed, 28 Jun 2017 11:11:54: 6000000 INFO @ Wed, 28 Jun 2017 11:11:56: 6000000 INFO @ Wed, 28 Jun 2017 11:11:57: 7000000 INFO @ Wed, 28 Jun 2017 11:12:01: 7000000 INFO @ Wed, 28 Jun 2017 11:12:03: 7000000 INFO @ Wed, 28 Jun 2017 11:12:04: 8000000 INFO @ Wed, 28 Jun 2017 11:12:09: 8000000 INFO @ Wed, 28 Jun 2017 11:12:10: 9000000 INFO @ Wed, 28 Jun 2017 11:12:11: 8000000 INFO @ Wed, 28 Jun 2017 11:12:16: 9000000 INFO @ Wed, 28 Jun 2017 11:12:17: 10000000 INFO @ Wed, 28 Jun 2017 11:12:18: 9000000 INFO @ Wed, 28 Jun 2017 11:12:23: 11000000 INFO @ Wed, 28 Jun 2017 11:12:23: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:12:23: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:12:23: #1 total tags in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:23: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:12:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:12:24: 10000000 INFO @ Wed, 28 Jun 2017 11:12:24: #1 tags after filtering in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:12:24: #1 finished! INFO @ Wed, 28 Jun 2017 11:12:24: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:12:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:12:24: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:12:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:12:24: Process for pairing-model is terminated! cat: SRX964222.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964222.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:12:26: 10000000 INFO @ Wed, 28 Jun 2017 11:12:31: 11000000 INFO @ Wed, 28 Jun 2017 11:12:31: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:12:31: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:12:31: #1 total tags in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:12:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:12:31: #1 tags after filtering in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:12:31: #1 finished! INFO @ Wed, 28 Jun 2017 11:12:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:12:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:12:32: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:12:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:12:32: Process for pairing-model is terminated! cat: SRX964222.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964222.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:12:33: 11000000 INFO @ Wed, 28 Jun 2017 11:12:33: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:12:33: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:12:33: #1 total tags in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:33: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:12:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:12:33: #1 tags after filtering in treatment: 11015742 INFO @ Wed, 28 Jun 2017 11:12:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:12:33: #1 finished! INFO @ Wed, 28 Jun 2017 11:12:33: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:12:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:12:34: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:12:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:12:34: Process for pairing-model is terminated! cat: SRX964222.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964222.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964222.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。