Job ID = 9163597 sra ファイルのダウンロード中... Completed: 823874K bytes transferred in 11 seconds (609577K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24422727 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964220/SRR1924327.sra Written 24422727 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:43 24422727 reads; of these: 24422727 (100.00%) were unpaired; of these: 1179313 (4.83%) aligned 0 times 18541838 (75.92%) aligned exactly 1 time 4701576 (19.25%) aligned >1 times 95.17% overall alignment rate Time searching: 00:04:43 Overall time: 00:04:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10247960 / 23243414 = 0.4409 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:13:02: # Command line: callpeak -t SRX964220.bam -f BAM -g 12100000 -n SRX964220.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964220.20 # format = BAM # ChIP-seq file = ['SRX964220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:02: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:02: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:02: # Command line: callpeak -t SRX964220.bam -f BAM -g 12100000 -n SRX964220.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964220.05 # format = BAM # ChIP-seq file = ['SRX964220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:02: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:02: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:02: # Command line: callpeak -t SRX964220.bam -f BAM -g 12100000 -n SRX964220.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964220.10 # format = BAM # ChIP-seq file = ['SRX964220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:02: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:02: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:09: 1000000 INFO @ Wed, 28 Jun 2017 11:13:09: 1000000 INFO @ Wed, 28 Jun 2017 11:13:09: 1000000 INFO @ Wed, 28 Jun 2017 11:13:16: 2000000 INFO @ Wed, 28 Jun 2017 11:13:16: 2000000 INFO @ Wed, 28 Jun 2017 11:13:16: 2000000 INFO @ Wed, 28 Jun 2017 11:13:22: 3000000 INFO @ Wed, 28 Jun 2017 11:13:22: 3000000 INFO @ Wed, 28 Jun 2017 11:13:23: 3000000 INFO @ Wed, 28 Jun 2017 11:13:28: 4000000 INFO @ Wed, 28 Jun 2017 11:13:29: 4000000 INFO @ Wed, 28 Jun 2017 11:13:29: 4000000 INFO @ Wed, 28 Jun 2017 11:13:35: 5000000 INFO @ Wed, 28 Jun 2017 11:13:36: 5000000 INFO @ Wed, 28 Jun 2017 11:13:37: 5000000 INFO @ Wed, 28 Jun 2017 11:13:43: 6000000 INFO @ Wed, 28 Jun 2017 11:13:45: 6000000 INFO @ Wed, 28 Jun 2017 11:13:45: 6000000 INFO @ Wed, 28 Jun 2017 11:13:51: 7000000 INFO @ Wed, 28 Jun 2017 11:13:53: 7000000 INFO @ Wed, 28 Jun 2017 11:13:53: 7000000 INFO @ Wed, 28 Jun 2017 11:13:58: 8000000 INFO @ Wed, 28 Jun 2017 11:14:01: 8000000 INFO @ Wed, 28 Jun 2017 11:14:01: 8000000 INFO @ Wed, 28 Jun 2017 11:14:06: 9000000 INFO @ Wed, 28 Jun 2017 11:14:09: 9000000 INFO @ Wed, 28 Jun 2017 11:14:09: 9000000 INFO @ Wed, 28 Jun 2017 11:14:13: 10000000 INFO @ Wed, 28 Jun 2017 11:14:17: 10000000 INFO @ Wed, 28 Jun 2017 11:14:17: 10000000 INFO @ Wed, 28 Jun 2017 11:14:20: 11000000 INFO @ Wed, 28 Jun 2017 11:14:25: 11000000 INFO @ Wed, 28 Jun 2017 11:14:25: 11000000 INFO @ Wed, 28 Jun 2017 11:14:28: 12000000 INFO @ Wed, 28 Jun 2017 11:14:33: 12000000 INFO @ Wed, 28 Jun 2017 11:14:33: 12000000 INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:35: #1 total tags in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:35: #1 tags after filtering in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:35: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:35: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:36: Process for pairing-model is terminated! cat: SRX964220.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964220.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:14:40: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:40: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:40: #1 total tags in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:40: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:40: #1 tags after filtering in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:40: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:40: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:41: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:41: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:41: #1 total tags in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:41: #1 tags after filtering in treatment: 12995454 INFO @ Wed, 28 Jun 2017 11:14:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:41: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:41: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:41: Process for pairing-model is terminated! cat: SRX964220.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964220.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:14:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:42: Process for pairing-model is terminated! cat: SRX964220.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964220.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964220.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。