Job ID = 9163595 sra ファイルのダウンロード中... Completed: 740430K bytes transferred in 9 seconds (615228K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22746218 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964218/SRR1924325.sra Written 22746218 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:03 22746218 reads; of these: 22746218 (100.00%) were unpaired; of these: 958079 (4.21%) aligned 0 times 18391323 (80.85%) aligned exactly 1 time 3396816 (14.93%) aligned >1 times 95.79% overall alignment rate Time searching: 00:04:03 Overall time: 00:04:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8890812 / 21788139 = 0.4081 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:11:42: # Command line: callpeak -t SRX964218.bam -f BAM -g 12100000 -n SRX964218.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964218.05 # format = BAM # ChIP-seq file = ['SRX964218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:42: # Command line: callpeak -t SRX964218.bam -f BAM -g 12100000 -n SRX964218.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964218.10 # format = BAM # ChIP-seq file = ['SRX964218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:42: # Command line: callpeak -t SRX964218.bam -f BAM -g 12100000 -n SRX964218.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964218.20 # format = BAM # ChIP-seq file = ['SRX964218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:11:42: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:11:42: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:11:49: 1000000 INFO @ Wed, 28 Jun 2017 11:11:49: 1000000 INFO @ Wed, 28 Jun 2017 11:11:49: 1000000 INFO @ Wed, 28 Jun 2017 11:11:55: 2000000 INFO @ Wed, 28 Jun 2017 11:11:56: 2000000 INFO @ Wed, 28 Jun 2017 11:11:56: 2000000 INFO @ Wed, 28 Jun 2017 11:12:02: 3000000 INFO @ Wed, 28 Jun 2017 11:12:04: 3000000 INFO @ Wed, 28 Jun 2017 11:12:04: 3000000 INFO @ Wed, 28 Jun 2017 11:12:10: 4000000 INFO @ Wed, 28 Jun 2017 11:12:11: 4000000 INFO @ Wed, 28 Jun 2017 11:12:12: 4000000 INFO @ Wed, 28 Jun 2017 11:12:17: 5000000 INFO @ Wed, 28 Jun 2017 11:12:19: 5000000 INFO @ Wed, 28 Jun 2017 11:12:19: 5000000 INFO @ Wed, 28 Jun 2017 11:12:24: 6000000 INFO @ Wed, 28 Jun 2017 11:12:27: 6000000 INFO @ Wed, 28 Jun 2017 11:12:27: 6000000 INFO @ Wed, 28 Jun 2017 11:12:31: 7000000 INFO @ Wed, 28 Jun 2017 11:12:34: 7000000 INFO @ Wed, 28 Jun 2017 11:12:35: 7000000 INFO @ Wed, 28 Jun 2017 11:12:38: 8000000 INFO @ Wed, 28 Jun 2017 11:12:42: 8000000 INFO @ Wed, 28 Jun 2017 11:12:42: 8000000 INFO @ Wed, 28 Jun 2017 11:12:46: 9000000 INFO @ Wed, 28 Jun 2017 11:12:50: 9000000 INFO @ Wed, 28 Jun 2017 11:12:50: 9000000 INFO @ Wed, 28 Jun 2017 11:12:53: 10000000 INFO @ Wed, 28 Jun 2017 11:12:57: 10000000 INFO @ Wed, 28 Jun 2017 11:12:58: 10000000 INFO @ Wed, 28 Jun 2017 11:13:00: 11000000 INFO @ Wed, 28 Jun 2017 11:13:05: 11000000 INFO @ Wed, 28 Jun 2017 11:13:05: 11000000 INFO @ Wed, 28 Jun 2017 11:13:07: 12000000 INFO @ Wed, 28 Jun 2017 11:13:12: 12000000 INFO @ Wed, 28 Jun 2017 11:13:12: 12000000 INFO @ Wed, 28 Jun 2017 11:13:13: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:13:13: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:13:13: #1 total tags in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:13: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:13:13: #1 tags after filtering in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:13:13: #1 finished! INFO @ Wed, 28 Jun 2017 11:13:13: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:13:14: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:13:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:13:14: Process for pairing-model is terminated! cat: SRX964218.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964218.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:13:18: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:13:18: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:13:18: #1 total tags in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:18: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:13:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:13:19: #1 tags after filtering in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:13:19: #1 finished! INFO @ Wed, 28 Jun 2017 11:13:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:13:19: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:13:19: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:13:19: #1 total tags in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:13:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:13:19: #1 tags after filtering in treatment: 12897327 INFO @ Wed, 28 Jun 2017 11:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:13:19: #1 finished! INFO @ Wed, 28 Jun 2017 11:13:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:13:19: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:13:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:13:19: Process for pairing-model is terminated! cat: SRX964218.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964218.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:13:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:13:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:13:20: Process for pairing-model is terminated! cat: SRX964218.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964218.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964218.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。