Job ID = 9163588 sra ファイルのダウンロード中... Completed: 669809K bytes transferred in 8 seconds (668703K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19899933 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964211/SRR1924318.sra Written 19899933 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 19899933 reads; of these: 19899933 (100.00%) were unpaired; of these: 1004976 (5.05%) aligned 0 times 15416281 (77.47%) aligned exactly 1 time 3478676 (17.48%) aligned >1 times 94.95% overall alignment rate Time searching: 00:03:55 Overall time: 00:03:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7619073 / 18894957 = 0.4032 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:09:07: # Command line: callpeak -t SRX964211.bam -f BAM -g 12100000 -n SRX964211.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964211.10 # format = BAM # ChIP-seq file = ['SRX964211.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:09:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:09:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:09:07: # Command line: callpeak -t SRX964211.bam -f BAM -g 12100000 -n SRX964211.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964211.20 # format = BAM # ChIP-seq file = ['SRX964211.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:09:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:09:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:09:07: # Command line: callpeak -t SRX964211.bam -f BAM -g 12100000 -n SRX964211.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964211.05 # format = BAM # ChIP-seq file = ['SRX964211.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:09:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:09:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:09:13: 1000000 INFO @ Wed, 28 Jun 2017 11:09:13: 1000000 INFO @ Wed, 28 Jun 2017 11:09:14: 1000000 INFO @ Wed, 28 Jun 2017 11:09:20: 2000000 INFO @ Wed, 28 Jun 2017 11:09:20: 2000000 INFO @ Wed, 28 Jun 2017 11:09:22: 2000000 INFO @ Wed, 28 Jun 2017 11:09:27: 3000000 INFO @ Wed, 28 Jun 2017 11:09:27: 3000000 INFO @ Wed, 28 Jun 2017 11:09:29: 3000000 INFO @ Wed, 28 Jun 2017 11:09:34: 4000000 INFO @ Wed, 28 Jun 2017 11:09:34: 4000000 INFO @ Wed, 28 Jun 2017 11:09:37: 4000000 INFO @ Wed, 28 Jun 2017 11:09:40: 5000000 INFO @ Wed, 28 Jun 2017 11:09:40: 5000000 INFO @ Wed, 28 Jun 2017 11:09:45: 5000000 INFO @ Wed, 28 Jun 2017 11:09:47: 6000000 INFO @ Wed, 28 Jun 2017 11:09:47: 6000000 INFO @ Wed, 28 Jun 2017 11:09:53: 6000000 INFO @ Wed, 28 Jun 2017 11:09:54: 7000000 INFO @ Wed, 28 Jun 2017 11:09:54: 7000000 INFO @ Wed, 28 Jun 2017 11:10:01: 8000000 INFO @ Wed, 28 Jun 2017 11:10:01: 7000000 INFO @ Wed, 28 Jun 2017 11:10:02: 8000000 INFO @ Wed, 28 Jun 2017 11:10:07: 9000000 INFO @ Wed, 28 Jun 2017 11:10:09: 8000000 INFO @ Wed, 28 Jun 2017 11:10:11: 9000000 INFO @ Wed, 28 Jun 2017 11:10:14: 10000000 INFO @ Wed, 28 Jun 2017 11:10:17: 9000000 INFO @ Wed, 28 Jun 2017 11:10:20: 10000000 INFO @ Wed, 28 Jun 2017 11:10:20: 11000000 INFO @ Wed, 28 Jun 2017 11:10:22: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:10:22: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:10:22: #1 total tags in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:22: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:10:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:10:22: #1 tags after filtering in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:10:22: #1 finished! INFO @ Wed, 28 Jun 2017 11:10:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:10:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:10:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:10:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:10:23: Process for pairing-model is terminated! cat: SRX964211.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964211.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:10:25: 10000000 INFO @ Wed, 28 Jun 2017 11:10:28: 11000000 INFO @ Wed, 28 Jun 2017 11:10:30: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:10:30: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:10:30: #1 total tags in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:30: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:10:31: #1 tags after filtering in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:10:31: #1 finished! INFO @ Wed, 28 Jun 2017 11:10:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:10:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:10:31: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:10:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:10:31: Process for pairing-model is terminated! cat: SRX964211.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964211.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:10:33: 11000000 INFO @ Wed, 28 Jun 2017 11:10:35: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:10:35: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:10:35: #1 total tags in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:10:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:10:35: #1 tags after filtering in treatment: 11275884 INFO @ Wed, 28 Jun 2017 11:10:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:10:35: #1 finished! INFO @ Wed, 28 Jun 2017 11:10:35: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:10:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:10:35: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:10:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:10:35: Process for pairing-model is terminated! cat: SRX964211.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964211.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964211.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。