Job ID = 9163587 sra ファイルのダウンロード中... Completed: 1122246K bytes transferred in 14 seconds (646105K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 33418181 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964210/SRR1924317.sra Written 33418181 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:53 33418181 reads; of these: 33418181 (100.00%) were unpaired; of these: 1678397 (5.02%) aligned 0 times 28799293 (86.18%) aligned exactly 1 time 2940491 (8.80%) aligned >1 times 94.98% overall alignment rate Time searching: 00:05:53 Overall time: 00:05:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16344800 / 31739784 = 0.5150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:14:14: # Command line: callpeak -t SRX964210.bam -f BAM -g 12100000 -n SRX964210.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964210.20 # format = BAM # ChIP-seq file = ['SRX964210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:14: # Command line: callpeak -t SRX964210.bam -f BAM -g 12100000 -n SRX964210.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964210.05 # format = BAM # ChIP-seq file = ['SRX964210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:14: # Command line: callpeak -t SRX964210.bam -f BAM -g 12100000 -n SRX964210.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964210.10 # format = BAM # ChIP-seq file = ['SRX964210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:14:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:14:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:14:21: 1000000 INFO @ Wed, 28 Jun 2017 11:14:22: 1000000 INFO @ Wed, 28 Jun 2017 11:14:22: 1000000 INFO @ Wed, 28 Jun 2017 11:14:29: 2000000 INFO @ Wed, 28 Jun 2017 11:14:29: 2000000 INFO @ Wed, 28 Jun 2017 11:14:29: 2000000 INFO @ Wed, 28 Jun 2017 11:14:36: 3000000 INFO @ Wed, 28 Jun 2017 11:14:36: 3000000 INFO @ Wed, 28 Jun 2017 11:14:36: 3000000 INFO @ Wed, 28 Jun 2017 11:14:42: 4000000 INFO @ Wed, 28 Jun 2017 11:14:43: 4000000 INFO @ Wed, 28 Jun 2017 11:14:43: 4000000 INFO @ Wed, 28 Jun 2017 11:14:49: 5000000 INFO @ Wed, 28 Jun 2017 11:14:49: 5000000 INFO @ Wed, 28 Jun 2017 11:14:49: 5000000 INFO @ Wed, 28 Jun 2017 11:14:54: 6000000 INFO @ Wed, 28 Jun 2017 11:14:55: 6000000 INFO @ Wed, 28 Jun 2017 11:14:55: 6000000 INFO @ Wed, 28 Jun 2017 11:15:00: 7000000 INFO @ Wed, 28 Jun 2017 11:15:01: 7000000 INFO @ Wed, 28 Jun 2017 11:15:01: 7000000 INFO @ Wed, 28 Jun 2017 11:15:06: 8000000 INFO @ Wed, 28 Jun 2017 11:15:07: 8000000 INFO @ Wed, 28 Jun 2017 11:15:08: 8000000 INFO @ Wed, 28 Jun 2017 11:15:12: 9000000 INFO @ Wed, 28 Jun 2017 11:15:13: 9000000 INFO @ Wed, 28 Jun 2017 11:15:14: 9000000 INFO @ Wed, 28 Jun 2017 11:15:18: 10000000 INFO @ Wed, 28 Jun 2017 11:15:19: 10000000 INFO @ Wed, 28 Jun 2017 11:15:20: 10000000 INFO @ Wed, 28 Jun 2017 11:15:24: 11000000 INFO @ Wed, 28 Jun 2017 11:15:25: 11000000 INFO @ Wed, 28 Jun 2017 11:15:27: 11000000 INFO @ Wed, 28 Jun 2017 11:15:30: 12000000 INFO @ Wed, 28 Jun 2017 11:15:31: 12000000 INFO @ Wed, 28 Jun 2017 11:15:34: 12000000 INFO @ Wed, 28 Jun 2017 11:15:37: 13000000 INFO @ Wed, 28 Jun 2017 11:15:37: 13000000 INFO @ Wed, 28 Jun 2017 11:15:40: 13000000 INFO @ Wed, 28 Jun 2017 11:15:43: 14000000 INFO @ Wed, 28 Jun 2017 11:15:43: 14000000 INFO @ Wed, 28 Jun 2017 11:15:46: 14000000 INFO @ Wed, 28 Jun 2017 11:15:49: 15000000 INFO @ Wed, 28 Jun 2017 11:15:49: 15000000 INFO @ Wed, 28 Jun 2017 11:15:51: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:51: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:51: #1 total tags in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:51: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:51: #1 tags after filtering in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:51: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:52: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:52: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:52: #1 total tags in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:52: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:52: #1 tags after filtering in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:52: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:52: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:52: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:52: Process for pairing-model is terminated! cat: SRX964210.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964210.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:15:53: 15000000 INFO @ Wed, 28 Jun 2017 11:15:53: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:53: Process for pairing-model is terminated! cat: SRX964210.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964210.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:15:55: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:15:55: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:15:55: #1 total tags in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:15:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:15:56: #1 tags after filtering in treatment: 15394984 INFO @ Wed, 28 Jun 2017 11:15:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:15:56: #1 finished! INFO @ Wed, 28 Jun 2017 11:15:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:15:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:15:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:15:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:15:56: Process for pairing-model is terminated! cat: SRX964210.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964210.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964210.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。