Job ID = 9163584 sra ファイルのダウンロード中... Completed: 946279K bytes transferred in 12 seconds (604177K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29030264 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964208/SRR1924315.sra Written 29030264 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 29030264 reads; of these: 29030264 (100.00%) were unpaired; of these: 1280744 (4.41%) aligned 0 times 23088758 (79.53%) aligned exactly 1 time 4660762 (16.05%) aligned >1 times 95.59% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13112244 / 27749520 = 0.4725 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:13:01: # Command line: callpeak -t SRX964208.bam -f BAM -g 12100000 -n SRX964208.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964208.05 # format = BAM # ChIP-seq file = ['SRX964208.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:01: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:01: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:01: # Command line: callpeak -t SRX964208.bam -f BAM -g 12100000 -n SRX964208.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964208.20 # format = BAM # ChIP-seq file = ['SRX964208.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:01: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:01: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:01: # Command line: callpeak -t SRX964208.bam -f BAM -g 12100000 -n SRX964208.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964208.10 # format = BAM # ChIP-seq file = ['SRX964208.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:13:01: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:13:01: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:13:08: 1000000 INFO @ Wed, 28 Jun 2017 11:13:08: 1000000 INFO @ Wed, 28 Jun 2017 11:13:08: 1000000 INFO @ Wed, 28 Jun 2017 11:13:14: 2000000 INFO @ Wed, 28 Jun 2017 11:13:14: 2000000 INFO @ Wed, 28 Jun 2017 11:13:14: 2000000 INFO @ Wed, 28 Jun 2017 11:13:21: 3000000 INFO @ Wed, 28 Jun 2017 11:13:21: 3000000 INFO @ Wed, 28 Jun 2017 11:13:21: 3000000 INFO @ Wed, 28 Jun 2017 11:13:27: 4000000 INFO @ Wed, 28 Jun 2017 11:13:27: 4000000 INFO @ Wed, 28 Jun 2017 11:13:27: 4000000 INFO @ Wed, 28 Jun 2017 11:13:34: 5000000 INFO @ Wed, 28 Jun 2017 11:13:34: 5000000 INFO @ Wed, 28 Jun 2017 11:13:34: 5000000 INFO @ Wed, 28 Jun 2017 11:13:40: 6000000 INFO @ Wed, 28 Jun 2017 11:13:40: 6000000 INFO @ Wed, 28 Jun 2017 11:13:41: 6000000 INFO @ Wed, 28 Jun 2017 11:13:47: 7000000 INFO @ Wed, 28 Jun 2017 11:13:47: 7000000 INFO @ Wed, 28 Jun 2017 11:13:47: 7000000 INFO @ Wed, 28 Jun 2017 11:13:53: 8000000 INFO @ Wed, 28 Jun 2017 11:13:53: 8000000 INFO @ Wed, 28 Jun 2017 11:13:54: 8000000 INFO @ Wed, 28 Jun 2017 11:13:59: 9000000 INFO @ Wed, 28 Jun 2017 11:13:59: 9000000 INFO @ Wed, 28 Jun 2017 11:14:00: 9000000 INFO @ Wed, 28 Jun 2017 11:14:06: 10000000 INFO @ Wed, 28 Jun 2017 11:14:06: 10000000 INFO @ Wed, 28 Jun 2017 11:14:07: 10000000 INFO @ Wed, 28 Jun 2017 11:14:12: 11000000 INFO @ Wed, 28 Jun 2017 11:14:12: 11000000 INFO @ Wed, 28 Jun 2017 11:14:13: 11000000 INFO @ Wed, 28 Jun 2017 11:14:18: 12000000 INFO @ Wed, 28 Jun 2017 11:14:18: 12000000 INFO @ Wed, 28 Jun 2017 11:14:20: 12000000 INFO @ Wed, 28 Jun 2017 11:14:25: 13000000 INFO @ Wed, 28 Jun 2017 11:14:25: 13000000 INFO @ Wed, 28 Jun 2017 11:14:26: 13000000 INFO @ Wed, 28 Jun 2017 11:14:31: 14000000 INFO @ Wed, 28 Jun 2017 11:14:31: 14000000 INFO @ Wed, 28 Jun 2017 11:14:33: 14000000 INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:35: #1 total tags in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:35: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:35: #1 total tags in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:36: #1 tags after filtering in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:36: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:36: #1 tags after filtering in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:36: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:36: Process for pairing-model is terminated! cat: SRX964208.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 11:14:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:36: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964208.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX964208.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964208.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:14:37: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:14:37: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:14:37: #1 total tags in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:37: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:14:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:14:37: #1 tags after filtering in treatment: 14637276 INFO @ Wed, 28 Jun 2017 11:14:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:14:37: #1 finished! INFO @ Wed, 28 Jun 2017 11:14:37: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:14:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:14:38: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:14:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:14:38: Process for pairing-model is terminated! cat: SRX964208.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964208.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964208.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。