Job ID = 9163582 sra ファイルのダウンロード中... Completed: 498982K bytes transferred in 8 seconds (474762K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 15245872 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964206/SRR1924313.sra Written 15245872 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:38 15245872 reads; of these: 15245872 (100.00%) were unpaired; of these: 812234 (5.33%) aligned 0 times 12312054 (80.76%) aligned exactly 1 time 2121584 (13.92%) aligned >1 times 94.67% overall alignment rate Time searching: 00:02:38 Overall time: 00:02:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5812906 / 14433638 = 0.4027 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:05:07: # Command line: callpeak -t SRX964206.bam -f BAM -g 12100000 -n SRX964206.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964206.10 # format = BAM # ChIP-seq file = ['SRX964206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:07: # Command line: callpeak -t SRX964206.bam -f BAM -g 12100000 -n SRX964206.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964206.20 # format = BAM # ChIP-seq file = ['SRX964206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:07: # Command line: callpeak -t SRX964206.bam -f BAM -g 12100000 -n SRX964206.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964206.05 # format = BAM # ChIP-seq file = ['SRX964206.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:07: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:07: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:15: 1000000 INFO @ Wed, 28 Jun 2017 11:05:15: 1000000 INFO @ Wed, 28 Jun 2017 11:05:15: 1000000 INFO @ Wed, 28 Jun 2017 11:05:22: 2000000 INFO @ Wed, 28 Jun 2017 11:05:23: 2000000 INFO @ Wed, 28 Jun 2017 11:05:23: 2000000 INFO @ Wed, 28 Jun 2017 11:05:30: 3000000 INFO @ Wed, 28 Jun 2017 11:05:31: 3000000 INFO @ Wed, 28 Jun 2017 11:05:31: 3000000 INFO @ Wed, 28 Jun 2017 11:05:38: 4000000 INFO @ Wed, 28 Jun 2017 11:05:39: 4000000 INFO @ Wed, 28 Jun 2017 11:05:39: 4000000 INFO @ Wed, 28 Jun 2017 11:05:45: 5000000 INFO @ Wed, 28 Jun 2017 11:05:47: 5000000 INFO @ Wed, 28 Jun 2017 11:05:47: 5000000 INFO @ Wed, 28 Jun 2017 11:05:53: 6000000 INFO @ Wed, 28 Jun 2017 11:05:55: 6000000 INFO @ Wed, 28 Jun 2017 11:05:55: 6000000 INFO @ Wed, 28 Jun 2017 11:06:01: 7000000 INFO @ Wed, 28 Jun 2017 11:06:03: 7000000 INFO @ Wed, 28 Jun 2017 11:06:03: 7000000 INFO @ Wed, 28 Jun 2017 11:06:08: 8000000 INFO @ Wed, 28 Jun 2017 11:06:11: 8000000 INFO @ Wed, 28 Jun 2017 11:06:11: 8000000 INFO @ Wed, 28 Jun 2017 11:06:13: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:06:13: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:06:13: #1 total tags in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:13: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:13: #1 tags after filtering in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:13: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:13: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:13: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:13: Process for pairing-model is terminated! cat: SRX964206.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964206.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:06:16: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:06:16: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:06:16: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:06:16: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:06:16: #1 total tags in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:16: #1 total tags in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:16: #1 tags after filtering in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:16: #1 tags after filtering in treatment: 8620732 INFO @ Wed, 28 Jun 2017 11:06:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:16: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:16: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:17: Process for pairing-model is terminated! cat: SRX964206.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964206.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:06:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:17: Process for pairing-model is terminated! cat: SRX964206.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964206.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964206.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。