Job ID = 9163581 sra ファイルのダウンロード中... Completed: 586485K bytes transferred in 9 seconds (520732K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20364453 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964205/SRR1924312.sra Written 20364453 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:36 20364453 reads; of these: 20364453 (100.00%) were unpaired; of these: 1316084 (6.46%) aligned 0 times 16149809 (79.30%) aligned exactly 1 time 2898560 (14.23%) aligned >1 times 93.54% overall alignment rate Time searching: 00:03:36 Overall time: 00:03:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7369437 / 19048369 = 0.3869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:07:03: # Command line: callpeak -t SRX964205.bam -f BAM -g 12100000 -n SRX964205.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964205.10 # format = BAM # ChIP-seq file = ['SRX964205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:07:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:07:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:07:03: # Command line: callpeak -t SRX964205.bam -f BAM -g 12100000 -n SRX964205.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964205.20 # format = BAM # ChIP-seq file = ['SRX964205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:07:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:07:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:07:03: # Command line: callpeak -t SRX964205.bam -f BAM -g 12100000 -n SRX964205.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964205.05 # format = BAM # ChIP-seq file = ['SRX964205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:07:03: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:07:03: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:07:10: 1000000 INFO @ Wed, 28 Jun 2017 11:07:10: 1000000 INFO @ Wed, 28 Jun 2017 11:07:10: 1000000 INFO @ Wed, 28 Jun 2017 11:07:16: 2000000 INFO @ Wed, 28 Jun 2017 11:07:16: 2000000 INFO @ Wed, 28 Jun 2017 11:07:17: 2000000 INFO @ Wed, 28 Jun 2017 11:07:23: 3000000 INFO @ Wed, 28 Jun 2017 11:07:23: 3000000 INFO @ Wed, 28 Jun 2017 11:07:23: 3000000 INFO @ Wed, 28 Jun 2017 11:07:30: 4000000 INFO @ Wed, 28 Jun 2017 11:07:30: 4000000 INFO @ Wed, 28 Jun 2017 11:07:30: 4000000 INFO @ Wed, 28 Jun 2017 11:07:36: 5000000 INFO @ Wed, 28 Jun 2017 11:07:36: 5000000 INFO @ Wed, 28 Jun 2017 11:07:37: 5000000 INFO @ Wed, 28 Jun 2017 11:07:42: 6000000 INFO @ Wed, 28 Jun 2017 11:07:42: 6000000 INFO @ Wed, 28 Jun 2017 11:07:43: 6000000 INFO @ Wed, 28 Jun 2017 11:07:48: 7000000 INFO @ Wed, 28 Jun 2017 11:07:48: 7000000 INFO @ Wed, 28 Jun 2017 11:07:50: 7000000 INFO @ Wed, 28 Jun 2017 11:07:55: 8000000 INFO @ Wed, 28 Jun 2017 11:07:55: 8000000 INFO @ Wed, 28 Jun 2017 11:07:57: 8000000 INFO @ Wed, 28 Jun 2017 11:08:01: 9000000 INFO @ Wed, 28 Jun 2017 11:08:01: 9000000 INFO @ Wed, 28 Jun 2017 11:08:04: 9000000 INFO @ Wed, 28 Jun 2017 11:08:08: 10000000 INFO @ Wed, 28 Jun 2017 11:08:08: 10000000 INFO @ Wed, 28 Jun 2017 11:08:10: 10000000 INFO @ Wed, 28 Jun 2017 11:08:15: 11000000 INFO @ Wed, 28 Jun 2017 11:08:15: 11000000 INFO @ Wed, 28 Jun 2017 11:08:17: 11000000 INFO @ Wed, 28 Jun 2017 11:08:19: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:08:19: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:08:19: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:08:19: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:08:19: #1 total tags in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:19: #1 total tags in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:20: #1 tags after filtering in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:20: #1 tags after filtering in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:20: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:20: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:20: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:20: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:20: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 11:08:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:20: Process for pairing-model is terminated! cat: SRX964205.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX964205.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964205.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964205.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:21: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:08:21: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:08:21: #1 total tags in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:22: #1 tags after filtering in treatment: 11678932 INFO @ Wed, 28 Jun 2017 11:08:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:22: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:22: Process for pairing-model is terminated! cat: SRX964205.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964205.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964205.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。