Job ID = 9163574 sra ファイルのダウンロード中... Completed: 556193K bytes transferred in 9 seconds (474097K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18102146 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964200/SRR1924307.sra Written 18102146 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:21 18102146 reads; of these: 18102146 (100.00%) were unpaired; of these: 1817226 (10.04%) aligned 0 times 14413867 (79.63%) aligned exactly 1 time 1871053 (10.34%) aligned >1 times 89.96% overall alignment rate Time searching: 00:03:21 Overall time: 00:03:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6175578 / 16284920 = 0.3792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:05:17: # Command line: callpeak -t SRX964200.bam -f BAM -g 12100000 -n SRX964200.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964200.10 # format = BAM # ChIP-seq file = ['SRX964200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:17: # Command line: callpeak -t SRX964200.bam -f BAM -g 12100000 -n SRX964200.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964200.20 # format = BAM # ChIP-seq file = ['SRX964200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:17: # Command line: callpeak -t SRX964200.bam -f BAM -g 12100000 -n SRX964200.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964200.05 # format = BAM # ChIP-seq file = ['SRX964200.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:05:17: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:05:17: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:05:25: 1000000 INFO @ Wed, 28 Jun 2017 11:05:25: 1000000 INFO @ Wed, 28 Jun 2017 11:05:25: 1000000 INFO @ Wed, 28 Jun 2017 11:05:32: 2000000 INFO @ Wed, 28 Jun 2017 11:05:32: 2000000 INFO @ Wed, 28 Jun 2017 11:05:32: 2000000 INFO @ Wed, 28 Jun 2017 11:05:39: 3000000 INFO @ Wed, 28 Jun 2017 11:05:40: 3000000 INFO @ Wed, 28 Jun 2017 11:05:40: 3000000 INFO @ Wed, 28 Jun 2017 11:05:46: 4000000 INFO @ Wed, 28 Jun 2017 11:05:47: 4000000 INFO @ Wed, 28 Jun 2017 11:05:47: 4000000 INFO @ Wed, 28 Jun 2017 11:05:53: 5000000 INFO @ Wed, 28 Jun 2017 11:05:55: 5000000 INFO @ Wed, 28 Jun 2017 11:05:55: 5000000 INFO @ Wed, 28 Jun 2017 11:06:00: 6000000 INFO @ Wed, 28 Jun 2017 11:06:03: 6000000 INFO @ Wed, 28 Jun 2017 11:06:03: 6000000 INFO @ Wed, 28 Jun 2017 11:06:10: 7000000 INFO @ Wed, 28 Jun 2017 11:06:12: 7000000 INFO @ Wed, 28 Jun 2017 11:06:12: 7000000 INFO @ Wed, 28 Jun 2017 11:06:20: 8000000 INFO @ Wed, 28 Jun 2017 11:06:21: 8000000 INFO @ Wed, 28 Jun 2017 11:06:21: 8000000 INFO @ Wed, 28 Jun 2017 11:06:29: 9000000 INFO @ Wed, 28 Jun 2017 11:06:29: 9000000 INFO @ Wed, 28 Jun 2017 11:06:30: 9000000 INFO @ Wed, 28 Jun 2017 11:06:38: 10000000 INFO @ Wed, 28 Jun 2017 11:06:38: 10000000 INFO @ Wed, 28 Jun 2017 11:06:39: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:06:39: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:06:39: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:06:39: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:06:39: #1 total tags in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:39: #1 total tags in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:39: #1 tags after filtering in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:39: #1 tags after filtering in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:39: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:39: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:39: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:39: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:40: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 11:06:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:40: Process for pairing-model is terminated! cat: SRX964200.05_peaks.narrowPeakcat: SRX964200.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964200.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.05_model.r'rm: cannot remove `SRX964200.10_*.xls': そのようなファイルやディレクトリはありません: そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:06:41: 10000000 INFO @ Wed, 28 Jun 2017 11:06:41: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 11:06:41: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 11:06:41: #1 total tags in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:06:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:06:42: #1 tags after filtering in treatment: 10109342 INFO @ Wed, 28 Jun 2017 11:06:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:06:42: #1 finished! INFO @ Wed, 28 Jun 2017 11:06:42: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:06:42: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:06:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:06:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:06:42: Process for pairing-model is terminated! cat: SRX964200.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964200.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964200.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。