Job ID = 9163572 sra ファイルのダウンロード中... Completed: 590425K bytes transferred in 8 seconds (538936K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 17994204 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964198/SRR1924305.sra Written 17994204 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:46 17994204 reads; of these: 17994204 (100.00%) were unpaired; of these: 817912 (4.55%) aligned 0 times 15548032 (86.41%) aligned exactly 1 time 1628260 (9.05%) aligned >1 times 95.45% overall alignment rate Time searching: 00:04:46 Overall time: 00:04:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7188287 / 17176292 = 0.4185 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:06:57: # Command line: callpeak -t SRX964198.bam -f BAM -g 12100000 -n SRX964198.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964198.10 # format = BAM # ChIP-seq file = ['SRX964198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:57: # Command line: callpeak -t SRX964198.bam -f BAM -g 12100000 -n SRX964198.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964198.20 # format = BAM # ChIP-seq file = ['SRX964198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:57: # Command line: callpeak -t SRX964198.bam -f BAM -g 12100000 -n SRX964198.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964198.05 # format = BAM # ChIP-seq file = ['SRX964198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:57: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:57: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:07:05: 1000000 INFO @ Wed, 28 Jun 2017 11:07:05: 1000000 INFO @ Wed, 28 Jun 2017 11:07:06: 1000000 INFO @ Wed, 28 Jun 2017 11:07:12: 2000000 INFO @ Wed, 28 Jun 2017 11:07:13: 2000000 INFO @ Wed, 28 Jun 2017 11:07:15: 2000000 INFO @ Wed, 28 Jun 2017 11:07:19: 3000000 INFO @ Wed, 28 Jun 2017 11:07:20: 3000000 INFO @ Wed, 28 Jun 2017 11:07:23: 3000000 INFO @ Wed, 28 Jun 2017 11:07:27: 4000000 INFO @ Wed, 28 Jun 2017 11:07:28: 4000000 INFO @ Wed, 28 Jun 2017 11:07:32: 4000000 INFO @ Wed, 28 Jun 2017 11:07:34: 5000000 INFO @ Wed, 28 Jun 2017 11:07:35: 5000000 INFO @ Wed, 28 Jun 2017 11:07:40: 5000000 INFO @ Wed, 28 Jun 2017 11:07:41: 6000000 INFO @ Wed, 28 Jun 2017 11:07:43: 6000000 INFO @ Wed, 28 Jun 2017 11:07:48: 7000000 INFO @ Wed, 28 Jun 2017 11:07:49: 6000000 INFO @ Wed, 28 Jun 2017 11:07:51: 7000000 INFO @ Wed, 28 Jun 2017 11:07:56: 8000000 INFO @ Wed, 28 Jun 2017 11:07:58: 7000000 INFO @ Wed, 28 Jun 2017 11:07:59: 8000000 INFO @ Wed, 28 Jun 2017 11:08:03: 9000000 INFO @ Wed, 28 Jun 2017 11:08:06: 8000000 INFO @ Wed, 28 Jun 2017 11:08:06: 9000000 INFO @ Wed, 28 Jun 2017 11:08:10: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:10: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:10: #1 total tags in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:10: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:10: #1 tags after filtering in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:10: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:10: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:11: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:11: Process for pairing-model is terminated! cat: SRX964198.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964198.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:14: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:14: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:14: #1 total tags in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:14: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:14: #1 tags after filtering in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:14: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:14: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:14: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:14: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:14: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:14: Process for pairing-model is terminated! cat: SRX964198.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964198.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:14: 9000000 INFO @ Wed, 28 Jun 2017 11:08:22: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:22: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:22: #1 total tags in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:22: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:22: #1 tags after filtering in treatment: 9988005 INFO @ Wed, 28 Jun 2017 11:08:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:22: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:22: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:22: Process for pairing-model is terminated! cat: SRX964198.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964198.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964198.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。