Job ID = 9163571 sra ファイルのダウンロード中... Completed: 702191K bytes transferred in 12 seconds (448546K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 21312629 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964197/SRR1924304.sra Written 21312629 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:50 21312629 reads; of these: 21312629 (100.00%) were unpaired; of these: 1021801 (4.79%) aligned 0 times 17635401 (82.75%) aligned exactly 1 time 2655427 (12.46%) aligned >1 times 95.21% overall alignment rate Time searching: 00:03:50 Overall time: 00:03:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7828979 / 20290828 = 0.3858 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:06:41: # Command line: callpeak -t SRX964197.bam -f BAM -g 12100000 -n SRX964197.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964197.05 # format = BAM # ChIP-seq file = ['SRX964197.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:41: # Command line: callpeak -t SRX964197.bam -f BAM -g 12100000 -n SRX964197.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964197.20 # format = BAM # ChIP-seq file = ['SRX964197.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:41: # Command line: callpeak -t SRX964197.bam -f BAM -g 12100000 -n SRX964197.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964197.10 # format = BAM # ChIP-seq file = ['SRX964197.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:06:41: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:06:41: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:06:48: 1000000 INFO @ Wed, 28 Jun 2017 11:06:48: 1000000 INFO @ Wed, 28 Jun 2017 11:06:48: 1000000 INFO @ Wed, 28 Jun 2017 11:06:55: 2000000 INFO @ Wed, 28 Jun 2017 11:06:56: 2000000 INFO @ Wed, 28 Jun 2017 11:06:56: 2000000 INFO @ Wed, 28 Jun 2017 11:07:02: 3000000 INFO @ Wed, 28 Jun 2017 11:07:04: 3000000 INFO @ Wed, 28 Jun 2017 11:07:04: 3000000 INFO @ Wed, 28 Jun 2017 11:07:09: 4000000 INFO @ Wed, 28 Jun 2017 11:07:11: 4000000 INFO @ Wed, 28 Jun 2017 11:07:11: 4000000 INFO @ Wed, 28 Jun 2017 11:07:16: 5000000 INFO @ Wed, 28 Jun 2017 11:07:19: 5000000 INFO @ Wed, 28 Jun 2017 11:07:19: 5000000 INFO @ Wed, 28 Jun 2017 11:07:24: 6000000 INFO @ Wed, 28 Jun 2017 11:07:27: 6000000 INFO @ Wed, 28 Jun 2017 11:07:27: 6000000 INFO @ Wed, 28 Jun 2017 11:07:31: 7000000 INFO @ Wed, 28 Jun 2017 11:07:34: 7000000 INFO @ Wed, 28 Jun 2017 11:07:34: 7000000 INFO @ Wed, 28 Jun 2017 11:07:38: 8000000 INFO @ Wed, 28 Jun 2017 11:07:42: 8000000 INFO @ Wed, 28 Jun 2017 11:07:42: 8000000 INFO @ Wed, 28 Jun 2017 11:07:45: 9000000 INFO @ Wed, 28 Jun 2017 11:07:49: 9000000 INFO @ Wed, 28 Jun 2017 11:07:49: 9000000 INFO @ Wed, 28 Jun 2017 11:07:52: 10000000 INFO @ Wed, 28 Jun 2017 11:07:57: 10000000 INFO @ Wed, 28 Jun 2017 11:07:57: 10000000 INFO @ Wed, 28 Jun 2017 11:07:59: 11000000 INFO @ Wed, 28 Jun 2017 11:08:04: 11000000 INFO @ Wed, 28 Jun 2017 11:08:04: 11000000 INFO @ Wed, 28 Jun 2017 11:08:06: 12000000 INFO @ Wed, 28 Jun 2017 11:08:09: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:09: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:09: #1 total tags in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:09: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:10: #1 tags after filtering in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:10: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:10: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:10: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:10: Process for pairing-model is terminated! cat: SRX964197.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964197.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:12: 12000000 INFO @ Wed, 28 Jun 2017 11:08:12: 12000000 INFO @ Wed, 28 Jun 2017 11:08:15: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:15: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:15: #1 total tags in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:15: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:15: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:08:15: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:08:15: #1 total tags in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:15: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:08:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:08:16: #1 tags after filtering in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:16: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:16: #1 tags after filtering in treatment: 12461849 INFO @ Wed, 28 Jun 2017 11:08:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:08:16: #1 finished! INFO @ Wed, 28 Jun 2017 11:08:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:08:16: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:16: Process for pairing-model is terminated! cat: SRX964197.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964197.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:08:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:08:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:08:17: Process for pairing-model is terminated! cat: SRX964197.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964197.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964197.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。