Job ID = 9163567 sra ファイルのダウンロード中... Completed: 1437373K bytes transferred in 15 seconds (760438K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 44367508 spots for /home/okishinya/chipatlas/results/sacCer3/SRX964193/SRR1924300.sra Written 44367508 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:23 44367508 reads; of these: 44367508 (100.00%) were unpaired; of these: 2525336 (5.69%) aligned 0 times 35461935 (79.93%) aligned exactly 1 time 6380237 (14.38%) aligned >1 times 94.31% overall alignment rate Time searching: 00:08:23 Overall time: 00:08:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 24032864 / 41842172 = 0.5744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 11:16:11: # Command line: callpeak -t SRX964193.bam -f BAM -g 12100000 -n SRX964193.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX964193.05 # format = BAM # ChIP-seq file = ['SRX964193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:16:11: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:16:11: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:16:11: # Command line: callpeak -t SRX964193.bam -f BAM -g 12100000 -n SRX964193.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX964193.20 # format = BAM # ChIP-seq file = ['SRX964193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:16:11: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:16:11: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:16:11: # Command line: callpeak -t SRX964193.bam -f BAM -g 12100000 -n SRX964193.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX964193.10 # format = BAM # ChIP-seq file = ['SRX964193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 11:16:11: #1 read tag files... INFO @ Wed, 28 Jun 2017 11:16:11: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 11:16:18: 1000000 INFO @ Wed, 28 Jun 2017 11:16:18: 1000000 INFO @ Wed, 28 Jun 2017 11:16:18: 1000000 INFO @ Wed, 28 Jun 2017 11:16:25: 2000000 INFO @ Wed, 28 Jun 2017 11:16:26: 2000000 INFO @ Wed, 28 Jun 2017 11:16:26: 2000000 INFO @ Wed, 28 Jun 2017 11:16:32: 3000000 INFO @ Wed, 28 Jun 2017 11:16:33: 3000000 INFO @ Wed, 28 Jun 2017 11:16:33: 3000000 INFO @ Wed, 28 Jun 2017 11:16:39: 4000000 INFO @ Wed, 28 Jun 2017 11:16:40: 4000000 INFO @ Wed, 28 Jun 2017 11:16:40: 4000000 INFO @ Wed, 28 Jun 2017 11:16:46: 5000000 INFO @ Wed, 28 Jun 2017 11:16:48: 5000000 INFO @ Wed, 28 Jun 2017 11:16:48: 5000000 INFO @ Wed, 28 Jun 2017 11:16:53: 6000000 INFO @ Wed, 28 Jun 2017 11:16:55: 6000000 INFO @ Wed, 28 Jun 2017 11:16:55: 6000000 INFO @ Wed, 28 Jun 2017 11:17:00: 7000000 INFO @ Wed, 28 Jun 2017 11:17:02: 7000000 INFO @ Wed, 28 Jun 2017 11:17:02: 7000000 INFO @ Wed, 28 Jun 2017 11:17:07: 8000000 INFO @ Wed, 28 Jun 2017 11:17:10: 8000000 INFO @ Wed, 28 Jun 2017 11:17:10: 8000000 INFO @ Wed, 28 Jun 2017 11:17:14: 9000000 INFO @ Wed, 28 Jun 2017 11:17:17: 9000000 INFO @ Wed, 28 Jun 2017 11:17:17: 9000000 INFO @ Wed, 28 Jun 2017 11:17:22: 10000000 INFO @ Wed, 28 Jun 2017 11:17:25: 10000000 INFO @ Wed, 28 Jun 2017 11:17:25: 10000000 INFO @ Wed, 28 Jun 2017 11:17:29: 11000000 INFO @ Wed, 28 Jun 2017 11:17:32: 11000000 INFO @ Wed, 28 Jun 2017 11:17:32: 11000000 INFO @ Wed, 28 Jun 2017 11:17:37: 12000000 INFO @ Wed, 28 Jun 2017 11:17:40: 12000000 INFO @ Wed, 28 Jun 2017 11:17:40: 12000000 INFO @ Wed, 28 Jun 2017 11:17:45: 13000000 INFO @ Wed, 28 Jun 2017 11:17:48: 13000000 INFO @ Wed, 28 Jun 2017 11:17:48: 13000000 INFO @ Wed, 28 Jun 2017 11:17:52: 14000000 INFO @ Wed, 28 Jun 2017 11:17:55: 14000000 INFO @ Wed, 28 Jun 2017 11:17:55: 14000000 INFO @ Wed, 28 Jun 2017 11:18:00: 15000000 INFO @ Wed, 28 Jun 2017 11:18:04: 15000000 INFO @ Wed, 28 Jun 2017 11:18:04: 15000000 INFO @ Wed, 28 Jun 2017 11:18:08: 16000000 INFO @ Wed, 28 Jun 2017 11:18:11: 16000000 INFO @ Wed, 28 Jun 2017 11:18:11: 16000000 INFO @ Wed, 28 Jun 2017 11:18:16: 17000000 INFO @ Wed, 28 Jun 2017 11:18:19: 17000000 INFO @ Wed, 28 Jun 2017 11:18:19: 17000000 INFO @ Wed, 28 Jun 2017 11:18:23: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:18:23: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:18:23: #1 total tags in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:23: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:18:23: #1 tags after filtering in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:23: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:23: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:24: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:24: Process for pairing-model is terminated! cat: SRX964193.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964193.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:18:25: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:18:25: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:18:25: #1 total tags in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:18:25: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 11:18:25: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 11:18:25: #1 total tags in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:25: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 11:18:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 11:18:26: #1 tags after filtering in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:26: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:26: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:26: #1 tags after filtering in treatment: 17809308 INFO @ Wed, 28 Jun 2017 11:18:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 11:18:26: #1 finished! INFO @ Wed, 28 Jun 2017 11:18:26: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 11:18:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 11:18:27: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:27: Process for pairing-model is terminated! cat: SRX964193.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964193.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 11:18:27: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 11:18:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 11:18:27: Process for pairing-model is terminated! cat: SRX964193.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX964193.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX964193.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。