Job ID = 14520924 SRX = SRX9431360 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10299996 spots for SRR12979652/SRR12979652.sra Written 10299996 spots for SRR12979652/SRR12979652.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 10299996 reads; of these: 10299996 (100.00%) were unpaired; of these: 339552 (3.30%) aligned 0 times 9090895 (88.26%) aligned exactly 1 time 869549 (8.44%) aligned >1 times 96.70% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2913432 / 9960444 = 0.2925 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:54: 1000000 INFO @ Sat, 15 Jan 2022 20:14:59: 2000000 INFO @ Sat, 15 Jan 2022 20:15:05: 3000000 INFO @ Sat, 15 Jan 2022 20:15:10: 4000000 INFO @ Sat, 15 Jan 2022 20:15:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:21: 6000000 INFO @ Sat, 15 Jan 2022 20:15:25: 1000000 INFO @ Sat, 15 Jan 2022 20:15:28: 7000000 INFO @ Sat, 15 Jan 2022 20:15:28: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:15:28: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:15:28: #1 total tags in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:15:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:28: #1 tags after filtering in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:15:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:15:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:15:31: 2000000 INFO @ Sat, 15 Jan 2022 20:15:36: 3000000 INFO @ Sat, 15 Jan 2022 20:15:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:47: 5000000 INFO @ Sat, 15 Jan 2022 20:15:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:53: 6000000 INFO @ Sat, 15 Jan 2022 20:15:55: 1000000 INFO @ Sat, 15 Jan 2022 20:15:59: 7000000 INFO @ Sat, 15 Jan 2022 20:15:59: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:15:59: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:15:59: #1 total tags in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:15:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:15:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:15:59: #1 tags after filtering in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:15:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:15:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:15:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:15:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:15:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:15:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:15:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:01: 2000000 INFO @ Sat, 15 Jan 2022 20:16:06: 3000000 INFO @ Sat, 15 Jan 2022 20:16:12: 4000000 INFO @ Sat, 15 Jan 2022 20:16:17: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:22: 6000000 INFO @ Sat, 15 Jan 2022 20:16:28: 7000000 INFO @ Sat, 15 Jan 2022 20:16:28: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:16:28: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:16:28: #1 total tags in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:16:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:28: #1 tags after filtering in treatment: 7047012 INFO @ Sat, 15 Jan 2022 20:16:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:16:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431360/SRX9431360.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。