Job ID = 14520890 SRX = SRX9431355 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15908407 spots for SRR12979648/SRR12979648.sra Written 15908407 spots for SRR12979648/SRR12979648.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:43 15908407 reads; of these: 15908407 (100.00%) were unpaired; of these: 814180 (5.12%) aligned 0 times 13468024 (84.66%) aligned exactly 1 time 1626203 (10.22%) aligned >1 times 94.88% overall alignment rate Time searching: 00:02:43 Overall time: 00:02:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6096579 / 15094227 = 0.4039 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:50: 1000000 INFO @ Sat, 15 Jan 2022 20:09:55: 2000000 INFO @ Sat, 15 Jan 2022 20:10:00: 3000000 INFO @ Sat, 15 Jan 2022 20:10:05: 4000000 INFO @ Sat, 15 Jan 2022 20:10:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:10:14: 6000000 INFO @ Sat, 15 Jan 2022 20:10:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:19: 7000000 INFO @ Sat, 15 Jan 2022 20:10:21: 1000000 INFO @ Sat, 15 Jan 2022 20:10:24: 8000000 INFO @ Sat, 15 Jan 2022 20:10:27: 2000000 INFO @ Sat, 15 Jan 2022 20:10:29: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:10:29: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:10:29: #1 total tags in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:10:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:29: #1 tags after filtering in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:10:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:10:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:10:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 33 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:10:32: 3000000 INFO @ Sat, 15 Jan 2022 20:10:38: 4000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 20:10:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:10:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:49: 6000000 INFO @ Sat, 15 Jan 2022 20:10:51: 1000000 INFO @ Sat, 15 Jan 2022 20:10:55: 7000000 INFO @ Sat, 15 Jan 2022 20:10:56: 2000000 INFO @ Sat, 15 Jan 2022 20:11:00: 8000000 INFO @ Sat, 15 Jan 2022 20:11:01: 3000000 INFO @ Sat, 15 Jan 2022 20:11:06: 4000000 INFO @ Sat, 15 Jan 2022 20:11:06: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:11:06: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:11:06: #1 total tags in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:11:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:11:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:11:06: #1 tags after filtering in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:11:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:11:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:11:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:11:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:11:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:11:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:11:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:11:11: 5000000 INFO @ Sat, 15 Jan 2022 20:11:16: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:11:21: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:11:25: 8000000 INFO @ Sat, 15 Jan 2022 20:11:30: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:11:30: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:11:30: #1 total tags in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:11:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:11:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:11:30: #1 tags after filtering in treatment: 8997648 INFO @ Sat, 15 Jan 2022 20:11:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:11:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:11:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:11:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:11:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:11:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:11:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431355/SRX9431355.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling