Job ID = 14520883 SRX = SRX9431351 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3911621 spots for SRR12979644/SRR12979644.sra Written 3911621 spots for SRR12979644/SRR12979644.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:26 3911621 reads; of these: 3911621 (100.00%) were unpaired; of these: 190461 (4.87%) aligned 0 times 3265079 (83.47%) aligned exactly 1 time 456081 (11.66%) aligned >1 times 95.13% overall alignment rate Time searching: 00:01:26 Overall time: 00:01:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 595299 / 3721160 = 0.1600 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:50: 1000000 INFO @ Sat, 15 Jan 2022 20:07:01: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:07:11: 3000000 INFO @ Sat, 15 Jan 2022 20:07:13: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:07:13: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:07:13: #1 total tags in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:07:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:13: #1 tags after filtering in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:07:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:07:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:13: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:07:13: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:21: 1000000 INFO @ Sat, 15 Jan 2022 20:07:31: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:07:42: 3000000 INFO @ Sat, 15 Jan 2022 20:07:44: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:07:44: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:07:44: #1 total tags in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:07:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:44: #1 tags after filtering in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:07:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:07:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:44: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:07:44: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:07:50: 1000000 INFO @ Sat, 15 Jan 2022 20:08:01: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:08:13: 3000000 INFO @ Sat, 15 Jan 2022 20:08:14: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:08:14: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:08:14: #1 total tags in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:08:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:14: #1 tags after filtering in treatment: 3125861 INFO @ Sat, 15 Jan 2022 20:08:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:08:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:14: #2 number of paired peaks: 41 WARNING @ Sat, 15 Jan 2022 20:08:14: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431351/SRX9431351.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。