Job ID = 14520843 SRX = SRX9431342 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13289696 spots for SRR12979635/SRR12979635.sra Written 13289696 spots for SRR12979635/SRR12979635.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:42 13289696 reads; of these: 13289696 (100.00%) were unpaired; of these: 707715 (5.33%) aligned 0 times 11061052 (83.23%) aligned exactly 1 time 1520929 (11.44%) aligned >1 times 94.67% overall alignment rate Time searching: 00:02:42 Overall time: 00:02:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3910900 / 12581981 = 0.3108 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:13: 1000000 INFO @ Sat, 15 Jan 2022 20:05:19: 2000000 INFO @ Sat, 15 Jan 2022 20:05:25: 3000000 INFO @ Sat, 15 Jan 2022 20:05:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:05:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:05:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:05:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:05:38: 5000000 INFO @ Sat, 15 Jan 2022 20:05:44: 1000000 INFO @ Sat, 15 Jan 2022 20:05:45: 6000000 INFO @ Sat, 15 Jan 2022 20:05:51: 2000000 INFO @ Sat, 15 Jan 2022 20:05:52: 7000000 INFO @ Sat, 15 Jan 2022 20:05:58: 3000000 INFO @ Sat, 15 Jan 2022 20:05:59: 8000000 INFO @ Sat, 15 Jan 2022 20:06:03: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:06:03: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:06:03: #1 total tags in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:06:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:03: #1 tags after filtering in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:06:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:06:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:06:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:06:05: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:12: 5000000 INFO @ Sat, 15 Jan 2022 20:06:14: 1000000 INFO @ Sat, 15 Jan 2022 20:06:19: 6000000 INFO @ Sat, 15 Jan 2022 20:06:21: 2000000 INFO @ Sat, 15 Jan 2022 20:06:26: 7000000 INFO @ Sat, 15 Jan 2022 20:06:28: 3000000 INFO @ Sat, 15 Jan 2022 20:06:32: 8000000 INFO @ Sat, 15 Jan 2022 20:06:35: 4000000 INFO @ Sat, 15 Jan 2022 20:06:37: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:06:37: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:06:37: #1 total tags in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:06:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:37: #1 tags after filtering in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:06:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:06:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:06:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:06:42: 5000000 INFO @ Sat, 15 Jan 2022 20:06:48: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:06:55: 7000000 INFO @ Sat, 15 Jan 2022 20:07:01: 8000000 INFO @ Sat, 15 Jan 2022 20:07:05: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:07:05: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:07:05: #1 total tags in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:07:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:07:05: #1 tags after filtering in treatment: 8671081 INFO @ Sat, 15 Jan 2022 20:07:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:07:05: #1 finished! INFO @ Sat, 15 Jan 2022 20:07:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:07:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:07:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:07:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:07:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431342/SRX9431342.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling