Job ID = 14520806 SRX = SRX9431333 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7636048 spots for SRR12979626/SRR12979626.sra Written 7636048 spots for SRR12979626/SRR12979626.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:16 7636048 reads; of these: 7636048 (100.00%) were unpaired; of these: 412467 (5.40%) aligned 0 times 6083272 (79.67%) aligned exactly 1 time 1140309 (14.93%) aligned >1 times 94.60% overall alignment rate Time searching: 00:02:16 Overall time: 00:02:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2357704 / 7223581 = 0.3264 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:58:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:58:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:58:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:58:59: 1000000 INFO @ Sat, 15 Jan 2022 19:59:09: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:59:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:59:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:59:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:59:20: 3000000 INFO @ Sat, 15 Jan 2022 19:59:29: 1000000 INFO @ Sat, 15 Jan 2022 19:59:30: 4000000 INFO @ Sat, 15 Jan 2022 19:59:39: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:59:39: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:59:39: #1 total tags in treatment: 4865877 INFO @ Sat, 15 Jan 2022 19:59:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:59:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:59:39: #1 tags after filtering in treatment: 4865877 INFO @ Sat, 15 Jan 2022 19:59:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:59:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:59:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:59:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:59:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:59:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:59:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:59:40: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:59:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:59:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:59:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:59:50: 3000000 INFO @ Sat, 15 Jan 2022 20:00:00: 1000000 INFO @ Sat, 15 Jan 2022 20:00:01: 4000000 INFO @ Sat, 15 Jan 2022 20:00:10: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:00:10: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:00:10: #1 total tags in treatment: 4865877 INFO @ Sat, 15 Jan 2022 20:00:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:00:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:00:10: #1 tags after filtering in treatment: 4865877 INFO @ Sat, 15 Jan 2022 20:00:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:00:10: #1 finished! INFO @ Sat, 15 Jan 2022 20:00:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:00:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:00:10: 2000000 INFO @ Sat, 15 Jan 2022 20:00:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:00:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:00:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:00:21: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:00:31: 4000000 INFO @ Sat, 15 Jan 2022 20:00:39: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:00:39: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:00:39: #1 total tags in treatment: 4865877 INFO @ Sat, 15 Jan 2022 20:00:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:00:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:00:40: #1 tags after filtering in treatment: 4865877 INFO @ Sat, 15 Jan 2022 20:00:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:00:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:00:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:00:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:00:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:00:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:00:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431333/SRX9431333.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling