Job ID = 14520804 SRX = SRX9431331 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7659514 spots for SRR12979624/SRR12979624.sra Written 7659514 spots for SRR12979624/SRR12979624.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 7659514 reads; of these: 7659514 (100.00%) were unpaired; of these: 362850 (4.74%) aligned 0 times 6423057 (83.86%) aligned exactly 1 time 873607 (11.41%) aligned >1 times 95.26% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2017020 / 7296664 = 0.2764 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:56:30: 1000000 INFO @ Sat, 15 Jan 2022 19:56:35: 2000000 INFO @ Sat, 15 Jan 2022 19:56:41: 3000000 INFO @ Sat, 15 Jan 2022 19:56:46: 4000000 INFO @ Sat, 15 Jan 2022 19:56:51: 5000000 INFO @ Sat, 15 Jan 2022 19:56:53: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:56:53: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:56:53: #1 total tags in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:56:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:53: #1 tags after filtering in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:56:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:56:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:53: #2 looking for paired plus/minus strand peaks... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:56:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:56:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:56:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:56:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:57:00: 1000000 INFO @ Sat, 15 Jan 2022 19:57:05: 2000000 INFO @ Sat, 15 Jan 2022 19:57:11: 3000000 INFO @ Sat, 15 Jan 2022 19:57:16: 4000000 INFO @ Sat, 15 Jan 2022 19:57:21: 5000000 INFO @ Sat, 15 Jan 2022 19:57:23: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:57:23: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:57:23: #1 total tags in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:57:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:57:23: #1 tags after filtering in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:57:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:57:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:57:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:57:23: #2 looking for paired plus/minus strand peaks... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:57:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:57:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:57:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:57:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:57:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:57:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:57:32: 1000000 INFO @ Sat, 15 Jan 2022 19:57:38: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:57:45: 3000000 INFO @ Sat, 15 Jan 2022 19:57:51: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:57:58: 5000000 INFO @ Sat, 15 Jan 2022 19:57:59: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:57:59: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:57:59: #1 total tags in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:57:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:57:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:57:59: #1 tags after filtering in treatment: 5279644 INFO @ Sat, 15 Jan 2022 19:57:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:57:59: #1 finished! INFO @ Sat, 15 Jan 2022 19:57:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:57:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:58:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:58:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:58:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431331/SRX9431331.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling