Job ID = 14520781 SRX = SRX9431329 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8479432 spots for SRR12979622/SRR12979622.sra Written 8479432 spots for SRR12979622/SRR12979622.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 8479432 reads; of these: 8479432 (100.00%) were unpaired; of these: 792404 (9.35%) aligned 0 times 6659698 (78.54%) aligned exactly 1 time 1027330 (12.12%) aligned >1 times 90.65% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2273308 / 7687028 = 0.2957 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:15: 1000000 INFO @ Sat, 15 Jan 2022 19:54:22: 2000000 INFO @ Sat, 15 Jan 2022 19:54:29: 3000000 INFO @ Sat, 15 Jan 2022 19:54:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:42: 5000000 INFO @ Sat, 15 Jan 2022 19:54:44: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:54:44: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:54:44: #1 total tags in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:54:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:44: #1 tags after filtering in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:54:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:44: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:54:46: 1000000 INFO @ Sat, 15 Jan 2022 19:54:53: 2000000 INFO @ Sat, 15 Jan 2022 19:55:00: 3000000 INFO @ Sat, 15 Jan 2022 19:55:07: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:55:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:55:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:55:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:55:13: 5000000 INFO @ Sat, 15 Jan 2022 19:55:16: 1000000 INFO @ Sat, 15 Jan 2022 19:55:16: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:55:16: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:55:16: #1 total tags in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:55:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:16: #1 tags after filtering in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:55:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:16: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:55:23: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:55:30: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:55:37: 4000000 INFO @ Sat, 15 Jan 2022 19:55:43: 5000000 INFO @ Sat, 15 Jan 2022 19:55:46: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:55:46: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:55:46: #1 total tags in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:55:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:46: #1 tags after filtering in treatment: 5413720 INFO @ Sat, 15 Jan 2022 19:55:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431329/SRX9431329.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling