Job ID = 14520773 SRX = SRX9431321 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10174816 spots for SRR12979614/SRR12979614.sra Written 10174816 spots for SRR12979614/SRR12979614.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:36 10174816 reads; of these: 10174816 (100.00%) were unpaired; of these: 1030655 (10.13%) aligned 0 times 7935815 (77.99%) aligned exactly 1 time 1208346 (11.88%) aligned >1 times 89.87% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3228114 / 9144161 = 0.3530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:18: 1000000 INFO @ Sat, 15 Jan 2022 19:54:24: 2000000 INFO @ Sat, 15 Jan 2022 19:54:30: 3000000 INFO @ Sat, 15 Jan 2022 19:54:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:41: 5000000 INFO @ Sat, 15 Jan 2022 19:54:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:48: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:54:48: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:54:48: #1 total tags in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:54:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:48: #1 tags after filtering in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:48: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:54:50: 1000000 INFO @ Sat, 15 Jan 2022 19:54:57: 2000000 INFO @ Sat, 15 Jan 2022 19:55:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:55:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:55:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:55:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:55:12: 4000000 INFO @ Sat, 15 Jan 2022 19:55:20: 5000000 INFO @ Sat, 15 Jan 2022 19:55:20: 1000000 INFO @ Sat, 15 Jan 2022 19:55:27: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:55:27: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:55:27: #1 total tags in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:55:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:27: #1 tags after filtering in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:27: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 19:55:28: 2000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:55:35: 3000000 INFO @ Sat, 15 Jan 2022 19:55:42: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:55:49: 5000000 INFO @ Sat, 15 Jan 2022 19:55:56: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:55:56: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:55:56: #1 total tags in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:55:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:56: #1 tags after filtering in treatment: 5916047 INFO @ Sat, 15 Jan 2022 19:55:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431321/SRX9431321.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling