Job ID = 14520756 SRX = SRX9431317 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8465160 spots for SRR12979610/SRR12979610.sra Written 8465160 spots for SRR12979610/SRR12979610.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 8465160 reads; of these: 8465160 (100.00%) were unpaired; of these: 690351 (8.16%) aligned 0 times 6854062 (80.97%) aligned exactly 1 time 920747 (10.88%) aligned >1 times 91.84% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2507479 / 7774809 = 0.3225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:36: 1000000 INFO @ Sat, 15 Jan 2022 19:52:42: 2000000 INFO @ Sat, 15 Jan 2022 19:52:48: 3000000 INFO @ Sat, 15 Jan 2022 19:52:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:53:00: 5000000 INFO @ Sat, 15 Jan 2022 19:53:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:53:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:53:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:02: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:53:02: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:53:02: #1 total tags in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:53:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:53:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:53:02: #1 tags after filtering in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:53:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:53:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:53:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:53:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:53:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:53:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:53:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:53:07: 1000000 INFO @ Sat, 15 Jan 2022 19:53:12: 2000000 INFO @ Sat, 15 Jan 2022 19:53:19: 3000000 INFO @ Sat, 15 Jan 2022 19:53:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:53:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:53:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:53:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:32: 5000000 INFO @ Sat, 15 Jan 2022 19:53:34: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:53:34: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:53:34: #1 total tags in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:53:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:53:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:53:34: #1 tags after filtering in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:53:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:53:34: #1 finished! INFO @ Sat, 15 Jan 2022 19:53:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:53:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:53:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:53:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:53:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:53:37: 1000000 INFO @ Sat, 15 Jan 2022 19:53:44: 2000000 INFO @ Sat, 15 Jan 2022 19:53:51: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:53:59: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:54:07: 5000000 INFO @ Sat, 15 Jan 2022 19:54:08: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 19:54:08: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 19:54:08: #1 total tags in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:54:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:08: #1 tags after filtering in treatment: 5267330 INFO @ Sat, 15 Jan 2022 19:54:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431317/SRX9431317.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling