Job ID = 14520755 SRX = SRX9431316 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6541435 spots for SRR12979609/SRR12979609.sra Written 6541435 spots for SRR12979609/SRR12979609.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:10 6541435 reads; of these: 6541435 (100.00%) were unpaired; of these: 493037 (7.54%) aligned 0 times 5293676 (80.93%) aligned exactly 1 time 754722 (11.54%) aligned >1 times 92.46% overall alignment rate Time searching: 00:01:10 Overall time: 00:01:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1528084 / 6048398 = 0.2526 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:04: 1000000 INFO @ Sat, 15 Jan 2022 19:51:10: 2000000 INFO @ Sat, 15 Jan 2022 19:51:16: 3000000 INFO @ Sat, 15 Jan 2022 19:51:21: 4000000 INFO @ Sat, 15 Jan 2022 19:51:24: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:51:24: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:51:24: #1 total tags in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:51:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:24: #1 tags after filtering in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:51:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:24: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:25: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 19:51:25: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:34: 1000000 INFO @ Sat, 15 Jan 2022 19:51:40: 2000000 INFO @ Sat, 15 Jan 2022 19:51:45: 3000000 INFO @ Sat, 15 Jan 2022 19:51:51: 4000000 INFO @ Sat, 15 Jan 2022 19:51:54: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:51:54: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:51:54: #1 total tags in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:51:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:54: #1 tags after filtering in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:51:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:54: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 19:51:54: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:06: 1000000 INFO @ Sat, 15 Jan 2022 19:52:13: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:52:21: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:52:28: 4000000 INFO @ Sat, 15 Jan 2022 19:52:32: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 19:52:32: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 19:52:32: #1 total tags in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:52:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:32: #1 tags after filtering in treatment: 4520314 INFO @ Sat, 15 Jan 2022 19:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:52:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:32: #2 number of paired peaks: 30 WARNING @ Sat, 15 Jan 2022 19:52:32: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431316/SRX9431316.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling