Job ID = 14520734 SRX = SRX9431306 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25078556 spots for SRR12979599/SRR12979599.sra Written 25078556 spots for SRR12979599/SRR12979599.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:31 25078556 reads; of these: 25078556 (100.00%) were unpaired; of these: 2345039 (9.35%) aligned 0 times 19848810 (79.15%) aligned exactly 1 time 2884707 (11.50%) aligned >1 times 90.65% overall alignment rate Time searching: 00:15:31 Overall time: 00:15:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10892520 / 22733517 = 0.4791 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:48: 1000000 INFO @ Sat, 15 Jan 2022 20:14:55: 2000000 INFO @ Sat, 15 Jan 2022 20:15:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:10: 4000000 INFO @ Sat, 15 Jan 2022 20:15:20: 5000000 INFO @ Sat, 15 Jan 2022 20:15:21: 1000000 INFO @ Sat, 15 Jan 2022 20:15:29: 6000000 INFO @ Sat, 15 Jan 2022 20:15:33: 2000000 INFO @ Sat, 15 Jan 2022 20:15:37: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:44: 3000000 INFO @ Sat, 15 Jan 2022 20:15:47: 8000000 INFO @ Sat, 15 Jan 2022 20:15:52: 1000000 INFO @ Sat, 15 Jan 2022 20:15:56: 4000000 INFO @ Sat, 15 Jan 2022 20:15:56: 9000000 INFO @ Sat, 15 Jan 2022 20:16:04: 2000000 INFO @ Sat, 15 Jan 2022 20:16:05: 10000000 INFO @ Sat, 15 Jan 2022 20:16:08: 5000000 INFO @ Sat, 15 Jan 2022 20:16:13: 11000000 INFO @ Sat, 15 Jan 2022 20:16:16: 3000000 INFO @ Sat, 15 Jan 2022 20:16:20: 6000000 INFO @ Sat, 15 Jan 2022 20:16:21: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:16:21: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:16:21: #1 total tags in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:16:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:21: #1 tags after filtering in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:16:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:16:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:28: 4000000 INFO @ Sat, 15 Jan 2022 20:16:32: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:40: 5000000 INFO @ Sat, 15 Jan 2022 20:16:43: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:16:50: 6000000 INFO @ Sat, 15 Jan 2022 20:16:54: 9000000 INFO @ Sat, 15 Jan 2022 20:17:01: 7000000 INFO @ Sat, 15 Jan 2022 20:17:05: 10000000 INFO @ Sat, 15 Jan 2022 20:17:12: 8000000 INFO @ Sat, 15 Jan 2022 20:17:16: 11000000 INFO @ Sat, 15 Jan 2022 20:17:23: 9000000 INFO @ Sat, 15 Jan 2022 20:17:24: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:17:24: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:17:24: #1 total tags in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:17:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:25: #1 tags after filtering in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:17:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:25: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:17:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:17:33: 10000000 INFO @ Sat, 15 Jan 2022 20:17:43: 11000000 INFO @ Sat, 15 Jan 2022 20:17:51: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 20:17:51: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 20:17:51: #1 total tags in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:17:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:51: #1 tags after filtering in treatment: 11840997 INFO @ Sat, 15 Jan 2022 20:17:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:17:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431306/SRX9431306.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling