Job ID = 14520732 SRX = SRX9431304 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10469553 spots for SRR12979597/SRR12979597.sra Written 10469553 spots for SRR12979597/SRR12979597.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:10 10469553 reads; of these: 10469553 (100.00%) were unpaired; of these: 928850 (8.87%) aligned 0 times 8294165 (79.22%) aligned exactly 1 time 1246538 (11.91%) aligned >1 times 91.13% overall alignment rate Time searching: 00:02:10 Overall time: 00:02:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3321714 / 9540703 = 0.3482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:35: 1000000 INFO @ Sat, 15 Jan 2022 19:47:40: 2000000 INFO @ Sat, 15 Jan 2022 19:47:45: 3000000 INFO @ Sat, 15 Jan 2022 19:47:50: 4000000 INFO @ Sat, 15 Jan 2022 19:47:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:00: 6000000 INFO @ Sat, 15 Jan 2022 19:48:02: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 19:48:02: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 19:48:02: #1 total tags in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:48:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:02: #1 tags after filtering in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:48:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:48:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:48:05: 1000000 INFO @ Sat, 15 Jan 2022 19:48:10: 2000000 INFO @ Sat, 15 Jan 2022 19:48:16: 3000000 INFO @ Sat, 15 Jan 2022 19:48:21: 4000000 INFO @ Sat, 15 Jan 2022 19:48:26: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:31: 6000000 INFO @ Sat, 15 Jan 2022 19:48:32: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 19:48:32: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 19:48:32: #1 total tags in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:48:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:32: #1 tags after filtering in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:48:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:48:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:48:35: 1000000 INFO @ Sat, 15 Jan 2022 19:48:40: 2000000 INFO @ Sat, 15 Jan 2022 19:48:46: 3000000 INFO @ Sat, 15 Jan 2022 19:48:51: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:48:56: 5000000 INFO @ Sat, 15 Jan 2022 19:49:01: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:49:02: #1 tag size is determined as 81 bps INFO @ Sat, 15 Jan 2022 19:49:02: #1 tag size = 81 INFO @ Sat, 15 Jan 2022 19:49:02: #1 total tags in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:49:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:02: #1 tags after filtering in treatment: 6218989 INFO @ Sat, 15 Jan 2022 19:49:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:02: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431304/SRX9431304.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling