Job ID = 14521181 SRX = SRX9431292 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5468236 spots for SRR12979586/SRR12979586.sra Written 5468236 spots for SRR12979586/SRR12979586.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:00 5468236 reads; of these: 5468236 (100.00%) were unpaired; of these: 382479 (6.99%) aligned 0 times 4287098 (78.40%) aligned exactly 1 time 798659 (14.61%) aligned >1 times 93.01% overall alignment rate Time searching: 00:01:00 Overall time: 00:01:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1346688 / 5085757 = 0.2648 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:36:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:18: 1000000 INFO @ Sat, 15 Jan 2022 20:36:23: 2000000 INFO @ Sat, 15 Jan 2022 20:36:28: 3000000 INFO @ Sat, 15 Jan 2022 20:36:31: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:36:31: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:36:31: #1 total tags in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:36:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:36:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:36:31: #1 tags after filtering in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:36:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:36:31: #1 finished! INFO @ Sat, 15 Jan 2022 20:36:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:36:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:36:32: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 20:36:32: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:36:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:36:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:36:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:36:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:36:49: 1000000 INFO @ Sat, 15 Jan 2022 20:36:54: 2000000 INFO @ Sat, 15 Jan 2022 20:37:00: 3000000 INFO @ Sat, 15 Jan 2022 20:37:04: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:37:04: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:37:04: #1 total tags in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:37:04: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:04: #1 tags after filtering in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:37:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:37:04: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:04: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 20:37:04: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:37:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:37:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:37:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:37:19: 1000000 INFO @ Sat, 15 Jan 2022 20:37:24: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:37:30: 3000000 INFO @ Sat, 15 Jan 2022 20:37:34: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:37:34: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:37:34: #1 total tags in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:37:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:37:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:37:34: #1 tags after filtering in treatment: 3739069 INFO @ Sat, 15 Jan 2022 20:37:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:37:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:37:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:37:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:37:34: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 20:37:34: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:37:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431292/SRX9431292.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。