Job ID = 14521179 SRX = SRX9431290 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16505935 spots for SRR12979584/SRR12979584.sra Written 16505935 spots for SRR12979584/SRR12979584.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:53 16505935 reads; of these: 16505935 (100.00%) were unpaired; of these: 773402 (4.69%) aligned 0 times 13774601 (83.45%) aligned exactly 1 time 1957932 (11.86%) aligned >1 times 95.31% overall alignment rate Time searching: 00:05:53 Overall time: 00:05:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8013362 / 15732533 = 0.5093 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:26: 1000000 INFO @ Sat, 15 Jan 2022 20:46:37: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:46:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:46:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:46:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:46:48: 3000000 INFO @ Sat, 15 Jan 2022 20:46:57: 1000000 INFO @ Sat, 15 Jan 2022 20:47:01: 4000000 INFO @ Sat, 15 Jan 2022 20:47:09: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:47:14: 5000000 INFO @ Sat, 15 Jan 2022 20:47:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:47:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:47:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:47:22: 3000000 INFO @ Sat, 15 Jan 2022 20:47:25: 6000000 INFO @ Sat, 15 Jan 2022 20:47:27: 1000000 INFO @ Sat, 15 Jan 2022 20:47:35: 4000000 INFO @ Sat, 15 Jan 2022 20:47:39: 7000000 INFO @ Sat, 15 Jan 2022 20:47:39: 2000000 INFO @ Sat, 15 Jan 2022 20:47:48: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:47:48: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:47:48: #1 total tags in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:47:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:47:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:47:48: #1 tags after filtering in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:47:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:47:48: #1 finished! INFO @ Sat, 15 Jan 2022 20:47:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:47:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:47:48: 5000000 INFO @ Sat, 15 Jan 2022 20:47:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:47:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:47:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:47:50: 3000000 INFO @ Sat, 15 Jan 2022 20:48:00: 4000000 INFO @ Sat, 15 Jan 2022 20:48:00: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:48:09: 5000000 INFO @ Sat, 15 Jan 2022 20:48:12: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:48:18: 6000000 INFO @ Sat, 15 Jan 2022 20:48:20: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:48:20: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:48:20: #1 total tags in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:48:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:20: #1 tags after filtering in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:48:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:48:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:48:27: 7000000 INFO @ Sat, 15 Jan 2022 20:48:33: #1 tag size is determined as 66 bps INFO @ Sat, 15 Jan 2022 20:48:33: #1 tag size = 66 INFO @ Sat, 15 Jan 2022 20:48:33: #1 total tags in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:48:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:48:33: #1 tags after filtering in treatment: 7719171 INFO @ Sat, 15 Jan 2022 20:48:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:48:33: #1 finished! INFO @ Sat, 15 Jan 2022 20:48:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:48:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:48:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:48:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:48:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431290/SRX9431290.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling