Job ID = 14521133 SRX = SRX9431289 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10098705 spots for SRR12979583/SRR12979583.sra Written 10098705 spots for SRR12979583/SRR12979583.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:36 10098705 reads; of these: 10098705 (100.00%) were unpaired; of these: 536373 (5.31%) aligned 0 times 8358683 (82.77%) aligned exactly 1 time 1203649 (11.92%) aligned >1 times 94.69% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4071230 / 9562332 = 0.4258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:33:35: 1000000 INFO @ Sat, 15 Jan 2022 20:33:42: 2000000 INFO @ Sat, 15 Jan 2022 20:33:49: 3000000 INFO @ Sat, 15 Jan 2022 20:33:56: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:33:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:33:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:33:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:03: 5000000 INFO @ Sat, 15 Jan 2022 20:34:06: 1000000 INFO @ Sat, 15 Jan 2022 20:34:06: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:34:06: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:34:06: #1 total tags in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:34:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:06: #1 tags after filtering in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:34:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:13: 2000000 INFO @ Sat, 15 Jan 2022 20:34:19: 3000000 INFO @ Sat, 15 Jan 2022 20:34:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:34:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:34:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:34:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:34:33: 5000000 INFO @ Sat, 15 Jan 2022 20:34:35: 1000000 INFO @ Sat, 15 Jan 2022 20:34:37: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:34:37: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:34:37: #1 total tags in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:34:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:34:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:34:37: #1 tags after filtering in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:34:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:34:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:34:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:34:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:34:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:34:42: 2000000 INFO @ Sat, 15 Jan 2022 20:34:48: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:34:54: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:35:00: 5000000 INFO @ Sat, 15 Jan 2022 20:35:02: #1 tag size is determined as 65 bps INFO @ Sat, 15 Jan 2022 20:35:02: #1 tag size = 65 INFO @ Sat, 15 Jan 2022 20:35:02: #1 total tags in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:35:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:35:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:35:02: #1 tags after filtering in treatment: 5491102 INFO @ Sat, 15 Jan 2022 20:35:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:35:02: #1 finished! INFO @ Sat, 15 Jan 2022 20:35:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:35:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:35:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:35:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:35:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431289/SRX9431289.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling