Job ID = 14521122 SRX = SRX9431280 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3496847 spots for SRR12979574/SRR12979574.sra Written 3496847 spots for SRR12979574/SRR12979574.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:50 3496847 reads; of these: 3496847 (100.00%) were unpaired; of these: 368105 (10.53%) aligned 0 times 2648523 (75.74%) aligned exactly 1 time 480219 (13.73%) aligned >1 times 89.47% overall alignment rate Time searching: 00:00:50 Overall time: 00:00:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 555413 / 3128742 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:29: 1000000 INFO @ Sat, 15 Jan 2022 20:30:38: 2000000 INFO @ Sat, 15 Jan 2022 20:30:42: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:30:42: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:30:42: #1 total tags in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:30:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:42: #1 tags after filtering in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:30:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:30:42: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:42: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:30:42: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:30:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:30:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:30:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:30:57: 1000000 INFO @ Sat, 15 Jan 2022 20:31:03: 2000000 INFO @ Sat, 15 Jan 2022 20:31:07: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:31:07: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:31:07: #1 total tags in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:31:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:07: #1 tags after filtering in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:31:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:31:07: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:07: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:31:07: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:31:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:31:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:31:27: 1000000 INFO @ Sat, 15 Jan 2022 20:31:33: 2000000 INFO @ Sat, 15 Jan 2022 20:31:36: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:31:36: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:31:36: #1 total tags in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:31:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:36: #1 tags after filtering in treatment: 2573329 INFO @ Sat, 15 Jan 2022 20:31:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:31:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:36: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:31:36: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431280/SRX9431280.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。