Job ID = 14521086 SRX = SRX9431279 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3682560 spots for SRR12979573/SRR12979573.sra Written 3682560 spots for SRR12979573/SRR12979573.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:43 3682560 reads; of these: 3682560 (100.00%) were unpaired; of these: 278899 (7.57%) aligned 0 times 3009793 (81.73%) aligned exactly 1 time 393868 (10.70%) aligned >1 times 92.43% overall alignment rate Time searching: 00:00:43 Overall time: 00:00:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 495187 / 3403661 = 0.1455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:25:46: 1000000 INFO @ Sat, 15 Jan 2022 20:25:52: 2000000 INFO @ Sat, 15 Jan 2022 20:25:57: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:25:57: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:25:57: #1 total tags in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:25:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:57: #1 tags after filtering in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:25:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:57: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:25:57: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:16: 1000000 INFO @ Sat, 15 Jan 2022 20:26:22: 2000000 INFO @ Sat, 15 Jan 2022 20:26:27: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:26:27: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:26:27: #1 total tags in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:26:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:27: #1 tags after filtering in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:26:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:27: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:27: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:26:27: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:47: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:26:54: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:27:01: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:27:01: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:27:01: #1 total tags in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:27:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:01: #1 tags after filtering in treatment: 2908474 INFO @ Sat, 15 Jan 2022 20:27:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:27:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:01: #2 number of paired peaks: 36 WARNING @ Sat, 15 Jan 2022 20:27:01: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431279/SRX9431279.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling