Job ID = 14521084 SRX = SRX9431277 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 5893208 spots for SRR12979571/SRR12979571.sra Written 5893208 spots for SRR12979571/SRR12979571.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:02 5893208 reads; of these: 5893208 (100.00%) were unpaired; of these: 433748 (7.36%) aligned 0 times 4844362 (82.20%) aligned exactly 1 time 615098 (10.44%) aligned >1 times 92.64% overall alignment rate Time searching: 00:01:02 Overall time: 00:01:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 992230 / 5459460 = 0.1817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:53: 1000000 INFO @ Sat, 15 Jan 2022 20:26:59: 2000000 INFO @ Sat, 15 Jan 2022 20:27:05: 3000000 INFO @ Sat, 15 Jan 2022 20:27:12: 4000000 INFO @ Sat, 15 Jan 2022 20:27:15: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:27:15: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:27:15: #1 total tags in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:27:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:15: #1 tags after filtering in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:27:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:27:15: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:15: #2 number of paired peaks: 12 WARNING @ Sat, 15 Jan 2022 20:27:15: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:24: 1000000 INFO @ Sat, 15 Jan 2022 20:27:30: 2000000 INFO @ Sat, 15 Jan 2022 20:27:36: 3000000 INFO @ Sat, 15 Jan 2022 20:27:42: 4000000 INFO @ Sat, 15 Jan 2022 20:27:44: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:27:44: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:27:44: #1 total tags in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:27:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:44: #1 tags after filtering in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:27:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:27:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:45: #2 number of paired peaks: 12 WARNING @ Sat, 15 Jan 2022 20:27:45: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:54: 1000000 INFO @ Sat, 15 Jan 2022 20:28:01: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:28:07: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:28:14: 4000000 INFO @ Sat, 15 Jan 2022 20:28:17: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:28:17: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:28:17: #1 total tags in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:28:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:28:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:28:18: #1 tags after filtering in treatment: 4467230 INFO @ Sat, 15 Jan 2022 20:28:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:28:18: #1 finished! INFO @ Sat, 15 Jan 2022 20:28:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:28:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:28:18: #2 number of paired peaks: 12 WARNING @ Sat, 15 Jan 2022 20:28:18: Too few paired peaks (12) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:28:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431277/SRX9431277.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling