Job ID = 14521082 SRX = SRX9431275 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 3566684 spots for SRR12979569/SRR12979569.sra Written 3566684 spots for SRR12979569/SRR12979569.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:38 3566684 reads; of these: 3566684 (100.00%) were unpaired; of these: 415297 (11.64%) aligned 0 times 2648966 (74.27%) aligned exactly 1 time 502421 (14.09%) aligned >1 times 88.36% overall alignment rate Time searching: 00:00:38 Overall time: 00:00:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 573250 / 3151387 = 0.1819 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:25:12: 1000000 INFO @ Sat, 15 Jan 2022 20:25:19: 2000000 INFO @ Sat, 15 Jan 2022 20:25:22: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 20:25:22: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 20:25:22: #1 total tags in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:25:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:22: #1 tags after filtering in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:25:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:22: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:25:22: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:25:41: 1000000 INFO @ Sat, 15 Jan 2022 20:25:46: 2000000 INFO @ Sat, 15 Jan 2022 20:25:49: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 20:25:49: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 20:25:49: #1 total tags in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:25:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:49: #1 tags after filtering in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:25:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:49: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:25:49: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:12: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:26:19: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:26:22: #1 tag size is determined as 69 bps INFO @ Sat, 15 Jan 2022 20:26:22: #1 tag size = 69 INFO @ Sat, 15 Jan 2022 20:26:22: #1 total tags in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:26:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:22: #1 tags after filtering in treatment: 2578137 INFO @ Sat, 15 Jan 2022 20:26:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:22: #2 number of paired peaks: 44 WARNING @ Sat, 15 Jan 2022 20:26:22: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431275/SRX9431275.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling