Job ID = 14521075 SRX = SRX9431270 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6018226 spots for SRR12979564/SRR12979564.sra Written 6018226 spots for SRR12979564/SRR12979564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:19 6018226 reads; of these: 6018226 (100.00%) were unpaired; of these: 512772 (8.52%) aligned 0 times 4704759 (78.18%) aligned exactly 1 time 800695 (13.30%) aligned >1 times 91.48% overall alignment rate Time searching: 00:01:19 Overall time: 00:01:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1072519 / 5505454 = 0.1948 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:25:57: 1000000 INFO @ Sat, 15 Jan 2022 20:26:04: 2000000 INFO @ Sat, 15 Jan 2022 20:26:10: 3000000 INFO @ Sat, 15 Jan 2022 20:26:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:19: #1 tag size is determined as 85 bps INFO @ Sat, 15 Jan 2022 20:26:19: #1 tag size = 85 INFO @ Sat, 15 Jan 2022 20:26:19: #1 total tags in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:26:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:19: #1 tags after filtering in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:26:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:20: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:26:20: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:27: 1000000 INFO @ Sat, 15 Jan 2022 20:26:34: 2000000 INFO @ Sat, 15 Jan 2022 20:26:40: 3000000 INFO @ Sat, 15 Jan 2022 20:26:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:50: #1 tag size is determined as 85 bps INFO @ Sat, 15 Jan 2022 20:26:50: #1 tag size = 85 INFO @ Sat, 15 Jan 2022 20:26:50: #1 total tags in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:26:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:50: #1 tags after filtering in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:50: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:50: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:26:50: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:57: 1000000 INFO @ Sat, 15 Jan 2022 20:27:04: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:27:11: 3000000 INFO @ Sat, 15 Jan 2022 20:27:19: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:27:22: #1 tag size is determined as 85 bps INFO @ Sat, 15 Jan 2022 20:27:22: #1 tag size = 85 INFO @ Sat, 15 Jan 2022 20:27:22: #1 total tags in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:27:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:22: #1 tags after filtering in treatment: 4432935 INFO @ Sat, 15 Jan 2022 20:27:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:27:22: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:22: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:27:22: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431270/SRX9431270.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling