Job ID = 14521024 SRX = SRX9431268 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6452138 spots for SRR12979562/SRR12979562.sra Written 6452138 spots for SRR12979562/SRR12979562.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:18 6452138 reads; of these: 6452138 (100.00%) were unpaired; of these: 636145 (9.86%) aligned 0 times 5036928 (78.07%) aligned exactly 1 time 779065 (12.07%) aligned >1 times 90.14% overall alignment rate Time searching: 00:01:18 Overall time: 00:01:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1083499 / 5815993 = 0.1863 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:20:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:20:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:20:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:20:39: 1000000 INFO @ Sat, 15 Jan 2022 20:20:45: 2000000 INFO @ Sat, 15 Jan 2022 20:20:51: 3000000 INFO @ Sat, 15 Jan 2022 20:20:56: 4000000 INFO @ Sat, 15 Jan 2022 20:21:01: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:21:01: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:21:01: #1 total tags in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:01: #1 tags after filtering in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:21:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:01: #2 looking for paired plus/minus strand peaks... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:01: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 20:21:01: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:09: 1000000 INFO @ Sat, 15 Jan 2022 20:21:15: 2000000 INFO @ Sat, 15 Jan 2022 20:21:21: 3000000 INFO @ Sat, 15 Jan 2022 20:21:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:32: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:21:32: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:21:32: #1 total tags in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:32: #1 tags after filtering in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:21:32: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:32: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 20:21:32: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:38: 1000000 INFO @ Sat, 15 Jan 2022 20:21:43: 2000000 INFO @ Sat, 15 Jan 2022 20:21:48: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:21:53: 4000000 INFO @ Sat, 15 Jan 2022 20:21:57: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:21:57: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:21:57: #1 total tags in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:57: #1 tags after filtering in treatment: 4732494 INFO @ Sat, 15 Jan 2022 20:21:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:21:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:57: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 20:21:57: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431268/SRX9431268.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。