Job ID = 14521022 SRX = SRX9431266 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7443934 spots for SRR12979560/SRR12979560.sra Written 7443934 spots for SRR12979560/SRR12979560.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:23 7443934 reads; of these: 7443934 (100.00%) were unpaired; of these: 559611 (7.52%) aligned 0 times 5913789 (79.44%) aligned exactly 1 time 970534 (13.04%) aligned >1 times 92.48% overall alignment rate Time searching: 00:01:23 Overall time: 00:01:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1402940 / 6884323 = 0.2038 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:43: 1000000 INFO @ Sat, 15 Jan 2022 20:21:49: 2000000 INFO @ Sat, 15 Jan 2022 20:21:54: 3000000 INFO @ Sat, 15 Jan 2022 20:21:59: 4000000 INFO @ Sat, 15 Jan 2022 20:22:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:07: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:22:07: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:22:07: #1 total tags in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:22:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:07: #1 tags after filtering in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:22:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:22:07: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:22:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:22:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:13: 1000000 INFO @ Sat, 15 Jan 2022 20:22:19: 2000000 INFO @ Sat, 15 Jan 2022 20:22:24: 3000000 INFO @ Sat, 15 Jan 2022 20:22:29: 4000000 INFO @ Sat, 15 Jan 2022 20:22:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:37: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:22:37: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:22:37: #1 total tags in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:22:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:37: #1 tags after filtering in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:22:37: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:22:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:22:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:45: 1000000 INFO @ Sat, 15 Jan 2022 20:22:52: 2000000 INFO @ Sat, 15 Jan 2022 20:22:58: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:23:05: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:23:11: 5000000 INFO @ Sat, 15 Jan 2022 20:23:14: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:23:14: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:23:14: #1 total tags in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:23:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:23:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:23:14: #1 tags after filtering in treatment: 5481383 INFO @ Sat, 15 Jan 2022 20:23:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:23:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:23:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:23:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:23:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:23:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:23:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431266/SRX9431266.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling