Job ID = 14521019 SRX = SRX9431263 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7644131 spots for SRR12979557/SRR12979557.sra Written 7644131 spots for SRR12979557/SRR12979557.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:44 7644131 reads; of these: 7644131 (100.00%) were unpaired; of these: 901143 (11.79%) aligned 0 times 5629903 (73.65%) aligned exactly 1 time 1113085 (14.56%) aligned >1 times 88.21% overall alignment rate Time searching: 00:02:44 Overall time: 00:02:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1564687 / 6742988 = 0.2320 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:52: 1000000 INFO @ Sat, 15 Jan 2022 20:24:02: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:12: 3000000 INFO @ Sat, 15 Jan 2022 20:24:21: 1000000 INFO @ Sat, 15 Jan 2022 20:24:23: 4000000 INFO @ Sat, 15 Jan 2022 20:24:31: 2000000 INFO @ Sat, 15 Jan 2022 20:24:34: 5000000 INFO @ Sat, 15 Jan 2022 20:24:35: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:24:35: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:24:35: #1 total tags in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:24:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:24:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:24:36: #1 tags after filtering in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:24:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:24:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:24:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:24:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:24:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:24:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:24:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:40: 3000000 INFO @ Sat, 15 Jan 2022 20:24:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:50: 4000000 INFO @ Sat, 15 Jan 2022 20:24:52: 1000000 INFO @ Sat, 15 Jan 2022 20:25:00: 5000000 INFO @ Sat, 15 Jan 2022 20:25:01: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:25:01: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:25:01: #1 total tags in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:25:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:01: #1 tags after filtering in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:25:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:03: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:25:14: 3000000 INFO @ Sat, 15 Jan 2022 20:25:24: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:25:34: 5000000 INFO @ Sat, 15 Jan 2022 20:25:35: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:25:35: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:25:35: #1 total tags in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:25:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:35: #1 tags after filtering in treatment: 5178301 INFO @ Sat, 15 Jan 2022 20:25:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:35: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431263/SRX9431263.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling