Job ID = 14521018 SRX = SRX9431262 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6786853 spots for SRR12979556/SRR12979556.sra Written 6786853 spots for SRR12979556/SRR12979556.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:34 6786853 reads; of these: 6786853 (100.00%) were unpaired; of these: 639620 (9.42%) aligned 0 times 5269712 (77.65%) aligned exactly 1 time 877521 (12.93%) aligned >1 times 90.58% overall alignment rate Time searching: 00:01:34 Overall time: 00:01:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1204036 / 6147233 = 0.1959 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:20:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:20:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:20:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:20:49: 1000000 INFO @ Sat, 15 Jan 2022 20:20:56: 2000000 INFO @ Sat, 15 Jan 2022 20:21:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:11: 4000000 INFO @ Sat, 15 Jan 2022 20:21:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:18: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:21:18: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:21:18: #1 total tags in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:21:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:19: #1 tags after filtering in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:21:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:21:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:19: 1000000 INFO @ Sat, 15 Jan 2022 20:21:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:21:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:25: 2000000 INFO @ Sat, 15 Jan 2022 20:21:31: 3000000 INFO @ Sat, 15 Jan 2022 20:21:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:21:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:21:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:21:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:21:43: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:21:43: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:21:43: #1 total tags in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:21:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:21:43: #1 tags after filtering in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:21:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:21:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:21:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:21:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:21:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:21:48: 1000000 INFO @ Sat, 15 Jan 2022 20:21:55: 2000000 INFO @ Sat, 15 Jan 2022 20:22:01: 3000000 INFO @ Sat, 15 Jan 2022 20:22:07: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:22:13: #1 tag size is determined as 84 bps INFO @ Sat, 15 Jan 2022 20:22:13: #1 tag size = 84 INFO @ Sat, 15 Jan 2022 20:22:13: #1 total tags in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:22:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:22:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:22:13: #1 tags after filtering in treatment: 4943197 INFO @ Sat, 15 Jan 2022 20:22:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:22:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:22:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:22:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:22:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:22:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:22:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431262/SRX9431262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。